PMID- 19350296
OWN - NLM
STAT- MEDLINE
DCOM- 20090727
LR  - 20211020
IS  - 1476-5535 (Electronic)
IS  - 1367-5435 (Linking)
VI  - 36
IP  - 7
DP  - 2009 Jul
TI  - Biomass granulation in an aerobic:anaerobic-enhanced biological phosphorus 
      removal process in a sequencing batch reactor with varying pH.
PG  - 885-93
LID - 10.1007/s10295-009-0566-3 [doi]
AB  - Long-term influences of different steady-state pH conditions on microbial 
      community composition were determined by fluorescence in situ hybridization 
      (FISH) in a laboratory scale reactor configured for enhanced biological 
      phosphorus removal (EBPR). Chemical profiles were consistent with shifts in 
      populations from polyphosphate-accumulating organisms (PAO) to 
      glycogen-accumulating organisms (GAO) when pH fell from pH 7.5 to 7.0 and then to 
      6.5. While biomass was both dispersed and flocculated at pH 7.5, almost complete 
      granulation occurred gradually after pH was dropped to 7.0, and these granules 
      increased in size as the pH was reduced further to 6.5. Reverting back to pH 7.5 
      led to granule breakdown and corresponding increases in anaerobic phosphate 
      release. Granules consisted almost entirely of Accumulibacter PAO cells, while 
      putative GAO populations were always present in small numbers. Results suggest 
      that low pH may contribute to granulation under these operational conditions. 
      While chemical profiles suggested the PAO:GAO balance was changing as pH fell, 
      FISH failed to reveal any marked corresponding increase in GAO abundances. 
      Instead, TEM evidence suggested the Accumulibacter PAO phenotype was becoming 
      more like that of a GAO. These data show how metabolically adaptable the 
      Accumulibacter PAO can be under anaerobic:aerobic conditions in being able to 
      cope with marked changes in plant conditions. They suggest that decreases in EBPR 
      capacity may not necessarily reflect shifts in community composition, but in the 
      existing population metabolism.
FAU - Ahn, Johwan
AU  - Ahn J
AD  - Biotechnology Research Centre, La Trobe University, Bendigo, VIC 3552, Australia.
FAU - McIlroy, Simon
AU  - McIlroy S
FAU - Schroeder, Sarah
AU  - Schroeder S
FAU - Seviour, Robert
AU  - Seviour R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090407
PL  - Germany
TA  - J Ind Microbiol Biotechnol
JT  - Journal of industrial microbiology & biotechnology
JID - 9705544
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Betaproteobacteria/*growth & development/*metabolism/ultrastructure
MH  - Biomass
MH  - *Bioreactors
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Electron, Transmission
MH  - Phosphorus/*metabolism
MH  - Water Purification/*methods
EDAT- 2009/04/08 09:00
MHDA- 2009/07/28 09:00
CRDT- 2009/04/08 09:00
PHST- 2009/01/14 00:00 [received]
PHST- 2009/03/16 00:00 [accepted]
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/07/28 09:00 [medline]
AID - 10.1007/s10295-009-0566-3 [doi]
PST - ppublish
SO  - J Ind Microbiol Biotechnol. 2009 Jul;36(7):885-93. doi: 
      10.1007/s10295-009-0566-3. Epub 2009 Apr 7.