PMID- 19350504
OWN - NLM
STAT- MEDLINE
DCOM- 20090728
LR  - 20090407
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 26
IP  - 2
DP  - 2009 Apr
TI  - [Identification of acute lymphoctic leukemia extramedullary relapse and PTLD 
      after allo-HSCT by monitoring sex chromosome chimeric status with FISH].
PG  - 147-50
LID - 10.3760/cma.j.issn.1003-9406.2009.02.006 [doi]
AB  - OBJECTIVE: To explore the role of monitoring sex chromosome chimeric status by 
      fluorescence in situ hybridization (FISH) in the identification of leukemic 
      extramedullary relapse and post-transplant lymphoproliferative disease (PTLD) in 
      acute lymphocytic leukemia (ALL) after allogeneic hematopoietic stem cell 
      transplantation (allo-HSCT). METHODS: Six ALL patients who received 
      sex-mismatched allo-HSCT and manifested extravisceral lymphadenectasis or local 
      lump were investigated. The sex chromosome chimeric status in tumor tissues and 
      bone marrows (BM) were monitored by FISH, and EBV-RNA in the tumor tissues were 
      detected by in situ hybridization (ISH). RESULTS: The sex chromosomes in BM of 
      all 6 patients were 100% donor-derived. Among the sex chromosome chimeric status 
      of tumor tissues, three patients were mainly recipient-derived, and the 
      percentage of sex chromosomes derived from recipients were 100%, 100% and 98.0%, 
      respectively, and then they were diagnosed leukemic extramedullary relapse. The 
      other 3 patients were donor-derived, the percentage was 98.5%, 96.0% and 91.5%, 
      respectively, and were diagnosed PTLD. EBV-RNA and latent membrane protein 
      (LMP-1) were positive in 2 patients with PTLD and negative in the other 4 
      patients. One patient with extramedullary relapse obtained partial remission, one 
      with PTLD gained complete remission, and the others died eventually after 
      therapy. CONCLUSION: Monitoring the sex chromosome chimeric status by FISH is an 
      effective method to distinguish leukemic extramedullary relapse from PTLD in ALL 
      received sex-mismatched donor HSCT.
FAU - Du, Xuan
AU  - Du X
AD  - Department of Hematology, Southern Medical University, Guangdong, People's 
      Republic of China.
FAU - Liu, Qi-fa
AU  - Liu QF
FAU - Zhang, Le-shi
AU  - Zhang LS
FAU - Song, Lan-lin
AU  - Song LL
FAU - Fan, Zhi-ping
AU  - Fan ZP
FAU - Xu, Bing
AU  - Xu B
FAU - Sun, Jing
AU  - Sun J
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Female
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoproliferative Disorders/pathology/*surgery
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology/physiopathology/*surgery
MH  - Recurrence
MH  - Sex Chromosomes/genetics/physiology
MH  - Transplantation Conditioning
MH  - Young Adult
EDAT- 2009/04/08 09:00
MHDA- 2009/07/29 09:00
CRDT- 2009/04/08 09:00
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/07/29 09:00 [medline]
AID - 940626031 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2009.02.006 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Apr;26(2):147-50. doi: 
      10.3760/cma.j.issn.1003-9406.2009.02.006.