PMID- 19350517
OWN - NLM
STAT- MEDLINE
DCOM- 20090728
LR  - 20090407
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 26
IP  - 2
DP  - 2009 Apr
TI  - [Fluorescence in situ hybridization studies on a myeloid leukemia patient with 
      ins(8;21)(q22;q22.1q22.3)].
PG  - 203-6
LID - 10.3760/cma.j.issn.1003-9406.2009.02.019 [doi]
AB  - OBJECTIVE: To report a case of acute myeloid leukemia (AML) with the insertion 
      (8;21)(q22;q22.1q22.3). A 33-year-old Chinese woman was referred to our hospital. 
      Hematologic data showed WBC 42.7 x 10(9)/L with monocytosis (monocyte counts 
      7.296 x 10(9)/L). Bone marrow aspirate was hypercellular with 4.5% monoblasts and 
      7.5% promonocytes. At first she was diagnosed with chronic myelomonocytic 
      leukemia (CMML) according to the FAB criteria. Initially the patient received 
      supportive care only, but her general condition rapidly became worse three months 
      later. The monoblasts and promonocytes in the bone marrow rose to 20.5%. After 
      two cycles of combined chemotherapy she obtained complete remission. METHODS: 
      Chromosome specimens were prepared by short-term culture of bone marrow cells. 
      Karyotype analysis was carried out by R-banding technique. Three fluorescence in 
      situ hybridization (FISH) analyses were performed using AML1-ETO dual color, dual 
      fusion probe, whole chromosome painting 8 and 21 probes, and cen-8 and Tel 21qter 
      probes, respectively. Reverse transcription polymerase chain reaction (RT-PCR) 
      assay for detecting the AML1-ETO fusion transcript was also performed. RESULTS: 
      Conventional cytogenetic analysis showed a karyotype of 46,XX,ins(8;21) 
      (q22;q22.1q22.3)[7]/46,XX[3]. FISH tests confirmed the insertion. RT-PCR analysis 
      detected the AML1-ETO fusion transcript. CONCLUSION: We consider that this 
      patient should be rediagnosed as acute myeloid leukemia according to the criteria 
      proposed by World Health Organization (WHO) and that FISH and RT-PCR play an 
      important role in verification of the ins(8;21).
FAU - Wu, Ya-fang
AU  - Wu YF
AD  - The First Affiliated Hospital of Soochow University, Jiangsu Institute of 
      Hematology, Suzhou, Jiangsu, People's Republic of China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Bai, Shu-xiao
AU  - Bai SX
FAU - Zhang, Jun
AU  - Zhang J
FAU - Yao, Li
AU  - Yao L
FAU - Wang, Yong
AU  - Wang Y
FAU - Qiu, Hui-ying
AU  - Qiu HY
FAU - Shen, Juan
AU  - Shen J
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Ma, Qin-fen
AU  - Ma QF
LA  - chi
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
SB  - IM
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 19
MH  - *Chromosomes, Human, Pair 8
MH  - Core Binding Factor Alpha 2 Subunit/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Karyotyping
MH  - Leukemia, Myeloid/*genetics
MH  - *Translocation, Genetic
EDAT- 2009/04/08 09:00
MHDA- 2009/07/29 09:00
CRDT- 2009/04/08 09:00
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/07/29 09:00 [medline]
AID - 940626044 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2009.02.019 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Apr;26(2):203-6. doi: 
      10.3760/cma.j.issn.1003-9406.2009.02.019.