PMID- 19350518
OWN - NLM
STAT- MEDLINE
DCOM- 20090728
LR  - 20151119
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 26
IP  - 2
DP  - 2009 Apr
TI  - [The application of fluorescence in situ hybridization in detecting chronic 
      myeloid leukemia].
PG  - 207-10
LID - 10.3760/cma.j.issn.1003-9406.2009.02.020 [doi]
AB  - OBJECTIVE: To evaluate the clinical significance of the application of 
      fluorescence in situ hybridization (FISH) in detecting chronic myeloid leukemia 
      (CML). METHODS: Chromosome preparation was made by using 24-hour culture. FISH 
      technique using dual color dual fusion (DC-DF) BCR/ABL probe was performed in all 
      158 cases and R-banding was also employed for karyotyping in some patients. 
      RESULTS: Among the 158 cases, 98 cases were Ph positive, of which 69 cases 
      (70.4%) were typical FISH pattern (1R1G2F), the other 29 cases (29.6%) showed 12 
      different types of atypical FISH pattern. The most frequent atypical patterns 
      found were 1R1G1F in 7 cases (7.1%), 2R1G1F in 5 cases (5.1%), 1R1G2F and 1R1G3F 
      in 4 cases (4.1%), 2R2G1F in 3 cases (3.1%). Karyotype analysis on 18 CML cases 
      with atypical FISH patterns demonstrated that the atypical FISH patterns were due 
      to variant translocation in 3 cases; the additional third signal was because of a 
      supernumerary Ph chromosome. The karyotyping results did not conform to FISH 
      results in four cases suggesting the conceivable mistakes in karyotyping. The 
      1R1G1F signal pattern seen in 3 cases with classical t(9;22) resulted from the 
      deletion of derivative chromosome 9. The 1R1G2F signal pattern detected in 40% to 
      64% of interphase cells of 3 cases without Ph chromosome by conventional 
      cytogenetic analysis suggested a submicroscopic translocation. Three cases 
      treated with Glivec or bone marrow transplantation showed normal karyotypes with 
      a small amount of BCR/ABL positive cells by FISH detection. CONCLUSION: FISH 
      technique is of great value for the diagnosis of CML and confirmation of variant 
      translocation, occult Ph translocation, derivative chromosome 9 deletion, 
      therapeutic effect of interferon and Glivec as well as detection of minimal 
      residual disease after bone marrow transplantation.
FAU - Qiu, Hai-rong
AU  - Qiu HR
AD  - Department of Hematology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu, People's Republic of China.
FAU - Miao, Kou-rong
AU  - Miao KR
FAU - Wang, Rong
AU  - Wang R
FAU - Qiao, Chun
AU  - Qiao C
FAU - Zhang, Jian-fu
AU  - Zhang JF
FAU - Zhang, Su-jiang
AU  - Zhang SJ
FAU - Qian, Si-xuan
AU  - Qian SX
FAU - Xu, Wei
AU  - Xu W
FAU - Li, Jian-yong
AU  - Li JY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/analysis
MH  - Chromosome Banding
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - Female
MH  - Fusion Proteins, bcr-abl
MH  - Gene Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic
MH  - Young Adult
EDAT- 2009/04/08 09:00
MHDA- 2009/07/29 09:00
CRDT- 2009/04/08 09:00
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/07/29 09:00 [medline]
AID - 940626045 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2009.02.020 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Apr;26(2):207-10. doi: 
      10.3760/cma.j.issn.1003-9406.2009.02.020.