PMID- 19350529
OWN - NLM
STAT- MEDLINE
DCOM- 20090901
LR  - 20090422
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 23
IP  - 10
DP  - 2009 May
TI  - Precolumn derivatization reagents for high-speed analysis of amines and amino 
      acids in biological fluid using liquid chromatography/electrospray ionization 
      tandem mass spectrometry.
PG  - 1483-92
LID - 10.1002/rcm.4026 [doi]
AB  - A rapid analytical method for amines and amino acids was developed, involving 
      derivatization with the novel reagent 3-aminopyridyl-N-hydroxysuccinimidyl 
      carbamate (APDS), followed by reversed-phase high-performance liquid 
      chromatography and electrospray ionization tandem mass spectrometry 
      (HPLC/ESI-MS/MS). More than 100 different analytes with amino groups, including 
      amino acids in biological fluids such as mammalian plasma, could be measured 
      within 10 min. The analytes were easily derivatized with APDS under the mild 
      conditions. Selective reaction monitoring of ESI-MS/MS in positive mode was 
      carried out to include the transitions of all of the protonated molecular ions of 
      analytes derivatized with APDS to the common fragment at m/z 121, which was 
      derived from the amino pyridyl moiety of the reagent. We evaluated the retention 
      time precision, the quantification limits, the linearity, the intra- and 
      inter-day precisions and the accuracy of 22 typical amino acids found in 
      biological fluids, by analyzing a standard amino acid mixture and rat plasma. The 
      intra-day relative standard deviations (RSDs) of the retention times of the 22 
      amino acids and their internal standards were within 0.9% and the inter-day RSDs 
      were less than 1.1%, except for asparagines, with an RSD of 1.9%. The intra-day 
      and inter-day RSDs of amino acid analyses in rat plasma were within 8.0% and 
      4.5%, respectively. The method, which facilitates the amino acid analysis of more 
      than 100 samples in a day, represents an alternative to traditional amino acid 
      analysis techniques, such as chromatography using postcolumn derivatization by 
      ninhydrin.
CI  - Copyright (c) 2009 John Wiley & Sons, Ltd.
FAU - Shimbo, Kazutaka
AU  - Shimbo K
AD  - Institute of Life Sciences, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, 
      Kawasaki 210-8681, Japan. kazutaka_shinbo@ajinomoto.com
FAU - Oonuki, Takashi
AU  - Oonuki T
FAU - Yahashi, Akihisa
AU  - Yahashi A
FAU - Hirayama, Kazuo
AU  - Hirayama K
FAU - Miyano, Hiroshi
AU  - Miyano H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (3-aminopyridyl-N-hydroxysuccinimidyl carbamate)
RN  - 0 (Amines)
RN  - 0 (Amino Acids)
RN  - 0 (Carbamates)
RN  - 0 (Succinates)
SB  - IM
MH  - Amines/blood/*chemistry
MH  - Amino Acids/blood/*chemistry
MH  - Animals
MH  - Carbamates/*chemistry
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/*methods
MH  - Succinates/*chemistry
EDAT- 2009/04/08 09:00
MHDA- 2009/09/02 06:00
CRDT- 2009/04/08 09:00
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/09/02 06:00 [medline]
AID - 10.1002/rcm.4026 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2009 May;23(10):1483-92. doi: 10.1002/rcm.4026.