PMID- 19350571
OWN - NLM
STAT- MEDLINE
DCOM- 20090901
LR  - 20211020
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 107
IP  - 3
DP  - 2009 Jun 1
TI  - HACE1: A novel repressor of RAR transcriptional activity.
PG  - 482-93
LID - 10.1002/jcb.22146 [doi]
AB  - The diverse biological actions of retinoic acid (RA) are mediated by RA receptors 
      (RARs) and retinoid X receptors (RXRs). While the coregulatory proteins that 
      interact with the ligand-dependent AF-2 in the E region are well studied, the 
      ligand-independent N-terminal AF-1 domain-interacting partners and their 
      influence(s) on the function of RARs are poorly understood. HECT domain and 
      Ankyrin repeat containing E3 ubiquitin-protein ligase (HACE1) was isolated as a 
      RARbeta(3) AB region interacting protein. HACE1 interacts with RARbeta(3) both in 
      in vitro GST pull-down and in cell-based coprecipitation assays. The interaction 
      sites map to the N terminus of RARbeta(3) and the C terminus of HACE1. HACE1 
      functionally represses the transcriptional activity of RARalpha(1), RARbeta 
      isoforms 1, 2, and 3, but not RARgamma(1) in luciferase reporter assays. In 
      addition, HACE1 represses the endogenous RAR-regulated genes CRABP II, RIG1 and 
      RARbeta(2), but not RAI3 in CAOV3 cells. Mutation of the putative catalytic 
      cysteine (C876 of LF HACE1), which is indispensable for its E3 ubiquitin ligase 
      activity, does not alter the repressive effect of HACE1 on the transcriptional 
      activity of RARbeta(3). On the other hand, HACE1 inhibits the RA dependent 
      degradation of RARbeta(3). It is possible that the repression of RAR-regulated 
      transcription by HACE1 is due to its ability to inhibit the RA-dependent 
      degradation of RARs.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Zhao, Jianhua
AU  - Zhao J
AD  - Department of Microbiology and Immunology, Temple University School of Medicine, 
      Philadelphia, Pennsylvania 19140, USA.
FAU - Zhang, Zhenping
AU  - Zhang Z
FAU - Vucetic, Zivjena
AU  - Vucetic Z
FAU - Soprano, Kenneth J
AU  - Soprano KJ
FAU - Soprano, Dianne Robert
AU  - Soprano DR
LA  - eng
GR  - R01 DK067558-02/DK/NIDDK NIH HHS/United States
GR  - DK67558/DK/NIDDK NIH HHS/United States
GR  - R01 CA064945/CA/NCI NIH HHS/United States
GR  - DK44517/DK/NIDDK NIH HHS/United States
GR  - R01 DK044517/DK/NIDDK NIH HHS/United States
GR  - CA64945/CA/NCI NIH HHS/United States
GR  - R01 DK067558/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Repressor Proteins)
RN  - EC 2.3.2.26 (HACE1 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Humans
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Receptors, Retinoic Acid/*metabolism
MH  - Repressor Proteins/genetics/*metabolism
MH  - *Transcription, Genetic
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
PMC - PMC2736627
MID - NIHMS137018
EDAT- 2009/04/08 09:00
MHDA- 2009/09/02 06:00
PMCR- 2009/09/02
CRDT- 2009/04/08 09:00
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/09/02 06:00 [medline]
PHST- 2009/09/02 00:00 [pmc-release]
AID - 10.1002/jcb.22146 [doi]
PST - ppublish
SO  - J Cell Biochem. 2009 Jun 1;107(3):482-93. doi: 10.1002/jcb.22146.