PMID- 19350658
OWN - NLM
STAT- MEDLINE
DCOM- 20091008
LR  - 20220310
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 27
IP  - 10
DP  - 2009 Oct
TI  - Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal 
      stem cells improve structural aspects of healing in equine flexor digitorum 
      superficialis tendons.
PG  - 1392-8
LID - 10.1002/jor.20887 [doi]
AB  - Tendinitis remains a catastrophic injury among athletes. Mesenchymal stem cells 
      (MSCs) have recently been investigated for use in the treatment of tendinitis. 
      Previous work has demonstrated the value of insulin-like growth factor-I (IGF-I) 
      to stimulate cellular proliferation and tendon fiber deposition in the core 
      lesion of tendinitis. This study examined the effects of MSCs, as well as IGF-I 
      gene-enhanced MSCs (AdIGF-MSCs) on tendon healing in vivo. Collagenase-induced 
      bilateral tendinitis lesions were created in equine flexor digitorum 
      superficialis tendons (SDFT). Tendons were treated with 10 x 10(6) MSCs or 10 x 
      10(6) AdIGF-MSCs. Control limbs were injected with 1 mL of phosphate-buffered 
      saline (PBS). Ultrasound examinations were performed at t = 0, 2, 4, 6, and 8 
      weeks. Horses were euthanized at 8 weeks and SDFTs were mechanically tested to 
      failure and evaluated for biochemical composition and histologic characteristics. 
      Expression of collagen types I and III, IGF-I, cartilage oligomeric matrix 
      protein (COMP), matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 
      (MMP-13), and aggrecanase-1 (ADAMTS-4) were similar in MSC and control tendons. 
      Both MSC and AdIGF-MSC injection resulted in significantly improved tendon 
      histological scores. These findings indicate a benefit to the use of MSCs and 
      AdIGF-MSCs for the treatment of tendinitis.
CI  - (c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Schnabel, Lauren V
AU  - Schnabel LV
AD  - Comparative Orthopaedics Laboratory, Department of Clinical Sciences, C3-187 
      Veterinary Medical Center, College of Veterinary Medicine, Cornell University, 
      Ithaca, New York 14853, USA.
FAU - Lynch, Maureen E
AU  - Lynch ME
FAU - van der Meulen, Marjolein C H
AU  - van der Meulen MC
FAU - Yeager, Amy E
AU  - Yeager AE
FAU - Kornatowski, Matthew A
AU  - Kornatowski MA
FAU - Nixon, Alan J
AU  - Nixon AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type III)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Matrilin Proteins)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.14 (Procollagen N-Endopeptidase)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.82 (ADAMTS4 Protein)
SB  - IM
MH  - ADAM Proteins/metabolism
MH  - ADAMTS4 Protein
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Cell- and Tissue-Based Therapy/methods/*veterinary
MH  - Collagen Type I/metabolism
MH  - Collagen Type III/metabolism
MH  - Extracellular Matrix Proteins/metabolism
MH  - Female
MH  - Genetic Therapy/methods/*veterinary
MH  - Glycoproteins/metabolism
MH  - Horse Diseases/metabolism/pathology/*therapy
MH  - Horses
MH  - Insulin-Like Growth Factor I/*genetics/metabolism
MH  - Male
MH  - Matrilin Proteins
MH  - Matrix Metalloproteinase 13/metabolism
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Mesenchymal Stem Cell Transplantation/methods/*veterinary
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Procollagen N-Endopeptidase/metabolism
MH  - Tendinopathy/metabolism/therapy/*veterinary
MH  - Tendons/*diagnostic imaging/metabolism/pathology
MH  - Ultrasonography
EDAT- 2009/04/08 09:00
MHDA- 2009/10/09 06:00
CRDT- 2009/04/08 09:00
PHST- 2009/04/08 09:00 [entrez]
PHST- 2009/04/08 09:00 [pubmed]
PHST- 2009/10/09 06:00 [medline]
AID - 10.1002/jor.20887 [doi]
PST - ppublish
SO  - J Orthop Res. 2009 Oct;27(10):1392-8. doi: 10.1002/jor.20887.