PMID- 19351881
OWN - NLM
STAT- MEDLINE
DCOM- 20090629
LR  - 20211020
IS  - 0021-9258 (Print)
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Linking)
VI  - 284
IP  - 22
DP  - 2009 May 29
TI  - Essential role of Hrs in endocytic recycling of full-length TrkB receptor but not 
      its isoform TrkB.T1.
PG  - 15126-36
LID - 10.1074/jbc.M809763200 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) signaling through its receptor, TrkB, 
      modulates survival, differentiation, and synaptic activity of neurons. Both 
      full-length TrkB (TrkB-FL) and its isoform T1 (TrkB.T1) receptors are expressed 
      in neurons; however, whether they follow the same endocytic pathway after BDNF 
      treatment is not known. In this study we report that TrkB-FL and TrkB.T1 
      receptors traverse divergent endocytic pathways after binding to BDNF. We provide 
      evidence that in neurons TrkB.T1 receptors predominantly recycle back to the cell 
      surface by a "default" mechanism. However, endocytosed TrkB-FL receptors recycle 
      to a lesser extent in a hepatocyte growth factor-regulated tyrosine kinase 
      substrate (Hrs)-dependent manner which relies on its tyrosine kinase activity. 
      The distinct role of Hrs in promoting recycling of internalized TrkB-FL receptors 
      is independent of its ubiquitin-interacting motif. Moreover, Hrs-sensitive 
      TrkB-FL recycling plays a role in BDNF-induced prolonged mitogen-activated 
      protein kinase (MAPK) activation. These observations provide evidence for 
      differential postendocytic sorting of TrkB-FL and TrkB.T1 receptors to alternate 
      intracellular pathways.
FAU - Huang, Shu-Hong
AU  - Huang SH
AD  - Department of Neurobiology, Key Laboratory of Medical Neurobiology, School of 
      Medicine, Jinan, Shandong 250012, China.
FAU - Zhao, Ling
AU  - Zhao L
FAU - Sun, Zong-Peng
AU  - Sun ZP
FAU - Li, Xue-Zhi
AU  - Li XZ
FAU - Geng, Zhao
AU  - Geng Z
FAU - Zhang, Kai-Di
AU  - Zhang KD
FAU - Chao, Moses V
AU  - Chao MV
FAU - Chen, Zhe-Yu
AU  - Chen ZY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090407
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Isoenzymes)
RN  - 0 (Ligands)
RN  - 0 (Phosphoproteins)
RN  - 0 (hepatocyte growth factor-regulated tyrosine kinase substrate)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Line
MH  - *Endocytosis/drug effects
MH  - Endosomal Sorting Complexes Required for Transport
MH  - Enzyme Activation/drug effects
MH  - Humans
MH  - Isoenzymes/metabolism
MH  - Kinetics
MH  - Ligands
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Models, Biological
MH  - Phosphoproteins/chemistry/*metabolism
MH  - Protein Processing, Post-Translational/drug effects
MH  - Protein Structure, Tertiary
MH  - Protein Transport/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/chemistry/*metabolism
MH  - Signal Transduction/drug effects
PMC - PMC2685694
EDAT- 2009/04/09 09:00
MHDA- 2009/06/30 09:00
PMCR- 2010/05/29
CRDT- 2009/04/09 09:00
PHST- 2009/04/09 09:00 [entrez]
PHST- 2009/04/09 09:00 [pubmed]
PHST- 2009/06/30 09:00 [medline]
PHST- 2010/05/29 00:00 [pmc-release]
AID - S0021-9258(19)82123-3 [pii]
AID - 15126 [pii]
AID - 10.1074/jbc.M809763200 [doi]
PST - ppublish
SO  - J Biol Chem. 2009 May 29;284(22):15126-36. doi: 10.1074/jbc.M809763200. Epub 2009 
      Apr 7.