PMID- 19351958
OWN - NLM
STAT- MEDLINE
DCOM- 20090727
LR  - 20240109
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 113
IP  - 23
DP  - 2009 Jun 4
TI  - Ligand of scavenger receptor class A indirectly induces maturation of human blood 
      dendritic cells via production of tumor necrosis factor-alpha.
PG  - 5839-47
LID - 10.1182/blood-2008-10-184796 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells for naive T 
      cells. In this study, scavenger receptor class A type I and type II (SR-A) were 
      shown to be expressed by peripheral blood DCs (PBDCs) and monocyte-derived DCs 
      (MDDCs). In addition, the binding of anti-SR-A antibody to these cells was lower 
      in the presence of fucoidan, an SR-A agonist. Treatment of these DCs with 
      fucoidan or anti-SR-A antibody markedly increased the surface expression of 
      costimulatory molecules CD83 and major histocompatibility complex class II on the 
      CD11c(high)CD123(low) myeloid subset of PBDCs. Furthermore, fucoidan-treated 
      PBDCs produced tumor necrosis factor-alpha (TNF-alpha) but not IL-12p70. In 
      addition, fucoidan-induced maturation was eliminated by pretreatment with 
      TNF-alpha-neutralizing antibody. Finally, interferon-gamma secretion and T-cell 
      proliferation were enhanced by coculture of T cells with fucoidan-matured PBDCs. 
      Specific inhibitors of p38 MAPK and glycogen synthase kinase 3 suppressed 
      TNF-alpha production and maturation of fucoidan-treated PBDCs. Moreover, MDDCs 
      lacking SR-A failed to up-regulate CD83 expression, TNF-alpha production, and 
      phosphorylation of p38 MAPK and glycogen synthase kinase 3-beta in the presence 
      of fucoidan. Taken together, these results suggest that ligation of SR-A leads to 
      induction of TNF-alpha, which subsequently induces PBDC maturation, thereby 
      leading to enhanced T-cell stimulatory capacity.
FAU - Jin, Jun-O
AU  - Jin JO
AD  - Department of Biochemistry, School of Medicine, Dong-A University, Busan, Korea.
FAU - Park, Hae-Young
AU  - Park HY
FAU - Xu, Qi
AU  - Xu Q
FAU - Park, Joo-In
AU  - Park JI
FAU - Zvyagintseva, Tatyana
AU  - Zvyagintseva T
FAU - Stonik, Valentin A
AU  - Stonik VA
FAU - Kwak, Jong-Young
AU  - Kwak JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090407
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Ligands)
RN  - 0 (Scavenger Receptors, Class A)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
MH  - Cell Differentiation/*immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Glycogen Synthase Kinase 3/metabolism
MH  - Humans
MH  - Ligands
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - RNA Interference
MH  - Scavenger Receptors, Class A/classification/genetics/*immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2009/04/09 09:00
MHDA- 2009/07/28 09:00
CRDT- 2009/04/09 09:00
PHST- 2009/04/09 09:00 [entrez]
PHST- 2009/04/09 09:00 [pubmed]
PHST- 2009/07/28 09:00 [medline]
AID - S0006-4971(20)37339-0 [pii]
AID - 10.1182/blood-2008-10-184796 [doi]
PST - ppublish
SO  - Blood. 2009 Jun 4;113(23):5839-47. doi: 10.1182/blood-2008-10-184796. Epub 2009 
      Apr 7.