PMID- 19352510
OWN - NLM
STAT- MEDLINE
DCOM- 20090706
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 4
DP  - 2009
TI  - A PKC-dependent recruitment of MMP-2 controls semaphorin-3A growth-promoting 
      effect in cortical dendrites.
PG  - e5099
LID - 10.1371/journal.pone.0005099 [doi]
LID - e5099
AB  - There is increasing evidence for a crucial role of proteases and 
      metalloproteinases during axon growth and guidance. In this context, we recently 
      described a functional link between the chemoattractive Sema3C and Matrix 
      metalloproteinase 3 (MMP3). Here, we provide data demonstrating the involvement 
      of MMP-2 to trigger the growth-promoting effect of Sema3A in cortical dendrites. 
      The in situ analysis of MMP-2 expression and activity is consistent with a 
      functional growth assay demonstrating in vitro that the pharmacological 
      inhibition of MMP-2 reduces the growth of cortical dendrites in response to 
      Sema3A. Hence, our results suggest that the selective recruitment and activation 
      of MMP-2 in response to Sema3A requires a PKC alpha dependent mechanism. 
      Altogether, we provide a second set of data supporting MMPs as effectors of the 
      growth-promoting effects of semaphorins, and we identify the potential signalling 
      pathway involved.
FAU - Gonthier, Bertrand
AU  - Gonthier B
AD  - INSERM U575, Physiopathologie du Systeme Nerveux, Strasbourg, France.
FAU - Koncina, Eric
AU  - Koncina E
FAU - Satkauskas, Saulius
AU  - Satkauskas S
FAU - Perraut, Martine
AU  - Perraut M
FAU - Roussel, Guy
AU  - Roussel G
FAU - Aunis, Dominique
AU  - Aunis D
FAU - Kapfhammer, Josef P
AU  - Kapfhammer JP
FAU - Bagnard, Dominique
AU  - Bagnard D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090408
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA Primers)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Semaphorin-3A)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - DNA Primers
MH  - *Dendrites
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase Inhibitors
MH  - Mice
MH  - Neurons/cytology/enzymology
MH  - Protease Inhibitors/pharmacology
MH  - Protein Kinase C/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Semaphorin-3A/*physiology
MH  - Up-Regulation
PMC - PMC2663036
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/04/09 09:00
MHDA- 2009/07/07 09:00
PMCR- 2009/04/08
CRDT- 2009/04/09 09:00
PHST- 2008/10/31 00:00 [received]
PHST- 2009/03/05 00:00 [accepted]
PHST- 2009/04/09 09:00 [entrez]
PHST- 2009/04/09 09:00 [pubmed]
PHST- 2009/07/07 09:00 [medline]
PHST- 2009/04/08 00:00 [pmc-release]
AID - 08-PONE-RA-07126R1 [pii]
AID - 10.1371/journal.pone.0005099 [doi]
PST - ppublish
SO  - PLoS One. 2009;4(4):e5099. doi: 10.1371/journal.pone.0005099. Epub 2009 Apr 8.