PMID- 19353689
OWN - NLM
STAT- MEDLINE
DCOM- 20091113
LR  - 20131121
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 35
IP  - 4
DP  - 2009 Jul-Aug
TI  - Cytoprotective effect of Artemisia capillaris fractions on oxidative 
      stress-induced apoptosis in V79 cells.
PG  - 380-8
LID - 10.1002/biof.35 [doi]
AB  - The objectives of this study were to determine the effects of Artemisia 
      capillaris on the viability, antioxidant status, and cytoprotective against 
      oxidative stress induced by hydrogen peroxide in V79 cells. Pretreatment with 
      ethyl acetate fraction increased 363% cell viability compared to the positive 
      control (PC). The ethyl acetate fraction of A. capillaris scavenged intracellular 
      reactive oxygen species (ROS) and increased the activities of cellular 
      antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase 
      (GSH-px), and catalase (CAT), and also increased glutathione (GSH) content. It 
      thereby prevented lipid peroxidation which was demonstrated by the inhibition of 
      the formation of thiobarbituric acid reactive substance (TBARS). Furthermore, 
      ethyl acetate fraction reduced the apoptotic cells and cell cycle arrest at G2/M 
      phase induced by H(2)O(2). These findings suggest that A. capillaris ethyl 
      acetate fraction protected V79 cells against H(2)O(2) damage, by enhancing the 
      antioxidative activity.
FAU - Hong, Jung-Hee
AU  - Hong JH
AD  - Traditional Microorganism Resources Center, Keimyung University, Dalseo-Gu, 
      Daegu, Republic of Korea.
FAU - Lee, In-Seon
AU  - Lee IS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Artemisia/*chemistry
MH  - Catalase/metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cricetinae
MH  - Cricetulus
MH  - Cytoprotection/*drug effects
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2009/04/09 09:00
MHDA- 2009/11/17 06:00
CRDT- 2009/04/09 09:00
PHST- 2009/04/09 09:00 [entrez]
PHST- 2009/04/09 09:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - 10.1002/biof.35 [doi]
PST - ppublish
SO  - Biofactors. 2009 Jul-Aug;35(4):380-8. doi: 10.1002/biof.35.