PMID- 19356111
OWN - NLM
STAT- MEDLINE
DCOM- 20090714
LR  - 20191111
IS  - 1874-0758 (Electronic)
IS  - 1872-3128 (Linking)
VI  - 3
IP  - 1
DP  - 2009 Jan
TI  - O-demethylation of dextromethorphan using microbial cultures.
PG  - 10-4
AB  - The potential of various microbes to metabolize the dextromethorphan (DXM) , a 
      CYP 2D6 substrate was studied to investigate similarities between microbial and 
      mammalian metabolism. Eight microbes were screened for their ability to 
      metabolize DXM in a manner comparable to humans with a view to develop 
      alternative systems to study human drug metabolism. The major metabolite of DXM 
      produced by Saccharomyces cerevisiae (NCIM 3090) was characterized by HPLC and 
      Liquid Chromatography Mass Spectrometry (LC/MS). DXM was biotransformed by 
      Saccharomyces cerevisiae into a major metabolite dextrorphan which is a known , 
      active metabolite of DXM in human. The microbial metabolism of DXM was similar to 
      the metabolism in mammals. The Saccharomyces cerevisiae could be used as a 
      suitable model strain in vitro to mimic CYP 2D6 metabolism and to produce 
      dextrorphan an active metabolite of DXM for further pharmacological, 
      toxicological studies.
FAU - Prasanthi, Samathoti
AU  - Prasanthi S
AD  - School of Pharmaceutical Sciences, Sri Padmavathi Mahila VisvaVidyalayam, 
      Tirupati - 517 502, A.P., India.
FAU - Vidyavathi, Maravajhala
AU  - Vidyavathi M
FAU - Prasad, Koganti V S R G
AU  - Prasad KV
FAU - Krishna, Devarakonda Rama
AU  - Krishna DR
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - Drug Metab Lett
JT  - Drug metabolism letters
JID - 101313587
RN  - 0 (Antitussive Agents)
RN  - 04B7QNO9WS (Dextrorphan)
RN  - 7355X3ROTS (Dextromethorphan)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP2D6)
SB  - IM
MH  - Antitussive Agents/*pharmacokinetics
MH  - Bacteria/*metabolism
MH  - Biotransformation
MH  - Chromatography, High Pressure Liquid
MH  - Cytochrome P-450 CYP2D6/metabolism
MH  - Dealkylation
MH  - Dextromethorphan/*pharmacokinetics
MH  - Dextrorphan/metabolism
MH  - Fungi/*metabolism
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Saccharomyces cerevisiae/metabolism
MH  - Spectrophotometry, Ultraviolet
MH  - Stereoisomerism
EDAT- 2009/04/10 09:00
MHDA- 2009/07/15 09:00
CRDT- 2009/04/10 09:00
PHST- 2009/04/10 09:00 [entrez]
PHST- 2009/04/10 09:00 [pubmed]
PHST- 2009/07/15 09:00 [medline]
AID - 10.2174/187231209787176326 [doi]
PST - ppublish
SO  - Drug Metab Lett. 2009 Jan;3(1):10-4. doi: 10.2174/187231209787176326.