PMID- 19357083
OWN - NLM
STAT- MEDLINE
DCOM- 20090713
LR  - 20211020
IS  - 0021-9258 (Print)
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Linking)
VI  - 284
IP  - 23
DP  - 2009 Jun 5
TI  - Lipopolysaccharide-induced maturation of bone marrow-derived dendritic cells is 
      regulated by notch signaling through the up-regulation of CXCR4.
PG  - 15993-6003
LID - 10.1074/jbc.M901144200 [doi]
AB  - Dendritic cells (DCs) are professional antigen presenting cells to initiate 
      immune response against pathogens, but mechanisms controlling the maturation of 
      DCs are unclear. Here we report that, in the absence of recombination signal 
      binding protein-Jkappa (RBP-J, the transcription factor mediating Notch 
      signaling), lipopolysaccharide-stimulated monocyte-derived DCs are arrested at a 
      developmental stage with few dendrites, low major histocompatibility complex II 
      (MHC II) expression, and reduced motility and antigen presentation ability. RBP-J 
      null DCs had lower expression of CXCR4. Transduction with a CXCR4-expressing 
      lentivirus rescued developmental arrest of RBP-J-deficient DCs. Activation of 
      Notch signaling in DCs up-regulated CXCR4 expression and increased the outgrowth 
      of dendrites and the expression of MHC II. These effects were abrogated by a 
      CXCR4 inhibitor. Therefore, Notch signaling is essential for DCs to transit from 
      a dendrite(low)MHC II(low) immature state into a dendrite(high)MHC II(high) 
      mature state, during the lipopolysaccharide-induced DC maturation, most likely 
      through the up-regulation of CXCR4.
FAU - Wang, Yao-Chun
AU  - Wang YC
AD  - State Key Laboratory of Cancer Biology, Department of Medical Genetics and 
      Developmental Biology, Tangdu Hospital, Fourth Military Medical University, Xi'an 
      710032, China.
FAU - Hu, Xing-Bin
AU  - Hu XB
FAU - He, Fei
AU  - He F
FAU - Feng, Fan
AU  - Feng F
FAU - Wang, Lin
AU  - Wang L
FAU - Li, Wei
AU  - Li W
FAU - Zhang, Ping
AU  - Zhang P
FAU - Li, Duan
AU  - Li D
FAU - Jia, Zhan-Sheng
AU  - Jia ZS
FAU - Liang, Ying-Min
AU  - Liang YM
FAU - Han, Hua
AU  - Han H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090407
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CXCR4 protein, mouse)
RN  - 0 (DNA Primers)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/immunology/physiology
MH  - Chemotaxis
MH  - DNA Primers
MH  - DNA Transposable Elements/genetics
MH  - Dendritic Cells/*cytology/drug effects/immunology
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/genetics
MH  - Humans
MH  - Hypersensitivity, Delayed/immunology
MH  - Lipopolysaccharides/*pharmacology
MH  - Lymphocyte Culture Test, Mixed
MH  - Lymphocytes/immunology
MH  - Mice
MH  - Receptors, CXCR4/*genetics/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Up-Regulation
PMC - PMC2708893
EDAT- 2009/04/10 09:00
MHDA- 2009/07/14 09:00
PMCR- 2010/06/05
CRDT- 2009/04/10 09:00
PHST- 2009/04/10 09:00 [entrez]
PHST- 2009/04/10 09:00 [pubmed]
PHST- 2009/07/14 09:00 [medline]
PHST- 2010/06/05 00:00 [pmc-release]
AID - S0021-9258(20)42572-4 [pii]
AID - M901144200 [pii]
AID - 10.1074/jbc.M901144200 [doi]
PST - ppublish
SO  - J Biol Chem. 2009 Jun 5;284(23):15993-6003. doi: 10.1074/jbc.M901144200. Epub 
      2009 Apr 7.