PMID- 19357701
OWN - NLM
STAT- MEDLINE
DCOM- 20090921
LR  - 20220318
IS  - 1476-5551 (Electronic)
IS  - 0887-6924 (Linking)
VI  - 23
IP  - 8
DP  - 2009 Aug
TI  - Mesenchymal stem cells from multiple myeloma patients display distinct genomic 
      profile as compared with those from normal donors.
PG  - 1515-27
LID - 10.1038/leu.2009.65 [doi]
AB  - It is an open question whether in multiple myeloma (MM) bone marrow stromal cells 
      contain genomic alterations, which may contribute to the pathogenesis of the 
      disease. We conducted an array-based comparative genomic hybridization 
      (array-CGH) analysis to compare the extent of unbalanced genomic alterations in 
      mesenchymal stem cells from 21 myeloma patients (MM-MSCs) and 12 normal donors 
      (ND-MSCs) after in vitro culture expansion. Whereas ND-MSCs were devoid of 
      genomic imbalances, several non-recurrent chromosomal gains and losses (>1 Mb 
      size) were detected in MM-MSCs. Using real-time reverse transcription PCR, we 
      found correlative deregulated expression for five genes encoded in regions for 
      which genomic imbalances were detected using array-CGH. In addition, only MM-MSCs 
      showed a specific pattern of 'hot-spot' regions with discrete (<1 Mb) genomic 
      alterations, some of which were confirmed using fluorescence in situ 
      hybridization (FISH). Within MM-MSC samples, unsupervised cluster analysis did 
      not correlate with particular clinicobiological features of MM patients. We also 
      explored whether cytogenetic abnormalities present in myelomatous plasma cells 
      (PCs) were shared by matching MSCs from the same patients using FISH. All MM-MSCs 
      were cytogenetically normal for the tested genomic alterations. Therefore we 
      cannot support a common progenitor for myeloma PCs and MSCs.
FAU - Garayoa, M
AU  - Garayoa M
AD  - Centro de Investigacion del Cancer, Instituto de Biologia Molecular y Celular del 
      Cancer, Universidad de Salamanca-CSIC, Salamanca, Spain. mgarayoa@usal.es
FAU - Garcia, J L
AU  - Garcia JL
FAU - Santamaria, C
AU  - Santamaria C
FAU - Garcia-Gomez, A
AU  - Garcia-Gomez A
FAU - Blanco, J F
AU  - Blanco JF
FAU - Pandiella, A
AU  - Pandiella A
FAU - Hernandez, J M
AU  - Hernandez JM
FAU - Sanchez-Guijo, F M
AU  - Sanchez-Guijo FM
FAU - del Canizo, M-C
AU  - del Canizo MC
FAU - Gutierrez, N C
AU  - Gutierrez NC
FAU - San Miguel, J F
AU  - San Miguel JF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090409
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology
MH  - Bone Marrow Cells/chemistry
MH  - Cell Lineage
MH  - Cells, Cultured/chemistry
MH  - Cluster Analysis
MH  - *Comparative Genomic Hybridization
MH  - Female
MH  - Gene Dosage
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mesenchymal Stem Cells/*chemistry
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics/pathology
MH  - Neoplastic Stem Cells/chemistry
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plasma Cells/chemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured/chemistry
EDAT- 2009/04/10 09:00
MHDA- 2009/09/22 06:00
CRDT- 2009/04/10 09:00
PHST- 2009/04/10 09:00 [entrez]
PHST- 2009/04/10 09:00 [pubmed]
PHST- 2009/09/22 06:00 [medline]
AID - leu200965 [pii]
AID - 10.1038/leu.2009.65 [doi]
PST - ppublish
SO  - Leukemia. 2009 Aug;23(8):1515-27. doi: 10.1038/leu.2009.65. Epub 2009 Apr 9.