PMID- 19359165 OWN - NLM STAT- MEDLINE DCOM- 20090806 LR - 20171116 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 100 IP - 17 DP - 2009 Sep TI - Ammonia biofiltration and community analysis of ammonia-oxidizing bacteria in biofilters. PG - 3869-76 LID - 10.1016/j.biortech.2009.03.021 [doi] AB - Biological removal of ammonia was investigated using compost and sludge as packing materials in laboratory-scale biofilters. The aim of this study is to characterize the composition of ammonia-oxidizing bacteria (AOB) in two biofilters designed to remove ammonia. Experimental tests and measurements included analysis of removal efficiency and metabolic products. The inlet concentration of ammonia applied was 20-100 mg m(-3). Removal efficiencies of BFC and BFS were in the range of 97-99% and 95-99%, respectively. Periodic analysis of the biofilter packing materials showed ammonia was removed from air stream by nitrification and by the improved absorption of NH(3) in the resultant acidity. Nitrate was the dominant product of NH(3) transformation. Changes in the composition of AOB were examined by using nested PCR, denaturing gradient gel electrophoresis (DGGE) and sequencing of DGGE bands. DGGE analysis of biofilter samples revealed that shifts in the community structure of AOB were observed in the experiment; however, the idle phase did not cause the structural shift of AOB. Phylogenetic analysis revealed the population of AOB showed Nitrosospira sp. remains the predominant population in BFC, while Nitrosomonas sp. is the predominant population in BFS. FAU - Jun, Yin AU - Jun Y AD - College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, PR China. yjun021@yahoo.com.cn FAU - Wenfeng, Xu AU - Wenfeng X LA - eng PT - Journal Article DEP - 20090408 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Nitrates) RN - 0 (Nitrites) RN - 0 (Sewage) RN - 0 (Soil) RN - 7664-41-7 (Ammonia) SB - IM MH - Ammonia/isolation & purification/*metabolism MH - Bacteria/*metabolism MH - Biodegradation, Environmental MH - Electrophoresis MH - Filtration/*methods MH - Hydrogen-Ion Concentration MH - Nitrates/analysis MH - Nitrites/analysis MH - Oxidation-Reduction MH - Sequence Analysis, DNA MH - Sewage/microbiology MH - Soil EDAT- 2009/04/11 09:00 MHDA- 2009/08/07 09:00 CRDT- 2009/04/11 09:00 PHST- 2008/11/23 00:00 [received] PHST- 2009/03/06 00:00 [revised] PHST- 2009/03/10 00:00 [accepted] PHST- 2009/04/11 09:00 [entrez] PHST- 2009/04/11 09:00 [pubmed] PHST- 2009/08/07 09:00 [medline] AID - S0960-8524(09)00276-4 [pii] AID - 10.1016/j.biortech.2009.03.021 [doi] PST - ppublish SO - Bioresour Technol. 2009 Sep;100(17):3869-76. doi: 10.1016/j.biortech.2009.03.021. Epub 2009 Apr 8.