PMID- 19359798
OWN - NLM
STAT- MEDLINE
DCOM- 20090608
LR  - 20211203
IS  - 1348-2246 (Electronic)
IS  - 0910-6340 (Linking)
VI  - 25
IP  - 4
DP  - 2009 Apr
TI  - Isolation and pharmacological characterization of fatty acids from saw palmetto 
      extract.
PG  - 553-7
AB  - Saw palmetto extract (SPE) has been widely used for the treatment of lower 
      urinary-tract symptoms secondary to benign prostatic hyperplasia. The mechanisms 
      of pharmacological effects of SPE include the inhibition of 5alpha-reductase, 
      anti-androgenic effects, anti-proliferative effects, and anti-inflammatory 
      effects. Previously, we showed that SPE bound actively to alpha(1)-adrenergic, 
      muscarinic and 1,4-dihydropyridine calcium channel (1,4-DHP) receptors in the 
      prostate and bladder of rats, whereas its active constituents have not been fully 
      clarified. The present investigation is aimed to identify the main active 
      components contained in hexane and diethyl ether extracts of SPE with the use of 
      column chromatography and preparative HPLC. Based on the binding activity with 
      alpha(1)-adrenergic, muscarinic, and 1,4-DHP receptors, both isolated oleic and 
      lauric acids were deduced to be active components. Authentic samples of oleic and 
      lauric acids also exhibited similar binding activities to these receptors as the 
      fatty acids isolated from SPE, consistent with our findings. In addition, oleic 
      and lauric acids inhibited 5alpha-reductase, possibly leading to therapeutic 
      effects against benign prostatic hyperplasia and related lower urinary-tract 
      symptoms.
FAU - Abe, Masayuki
AU  - Abe M
AD  - Department of Pharmacokinetics and Pharmacodynamics, and Global Center of 
      Excellence Program, School of Pharmaceutical Sciences, University of Shizuoka, 
      Suruga, Shizuoka 422-8526, Japan.
FAU - Ito, Yoshihiko
AU  - Ito Y
FAU - Suzuki, Asahi
AU  - Suzuki A
FAU - Onoue, Satomi
AU  - Onoue S
FAU - Noguchi, Hiroshi
AU  - Noguchi H
FAU - Yamada, Shizuo
AU  - Yamada S
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Anal Sci
JT  - Analytical sciences : the international journal of the Japan Society for 
      Analytical Chemistry
JID - 8511078
RN  - 0 (Calcium Channels)
RN  - 0 (Dihydropyridines)
RN  - 0 (Fatty Acids)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptors, Adrenergic, alpha-1)
RN  - 0 (Receptors, Muscarinic)
RN  - 7M8K3P6I89 (1,4-dihydropyridine)
RN  - EC 1.3.1.22 (Cholestenone 5 alpha-Reductase)
RN  - J7WWH9M8QS (saw palmetto extract)
SB  - IM
MH  - Animals
MH  - Calcium Channels/metabolism
MH  - Cholestenone 5 alpha-Reductase/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Dihydropyridines/metabolism
MH  - Fatty Acids/*isolation & purification/metabolism/*pharmacology/therapeutic use
MH  - Male
MH  - Plant Extracts/*chemistry
MH  - Prostatic Hyperplasia/drug therapy/metabolism
MH  - Rats
MH  - Receptors, Adrenergic, alpha-1/metabolism
MH  - Receptors, Muscarinic/metabolism
MH  - Serenoa
MH  - Urologic Diseases/drug therapy/metabolism
EDAT- 2009/04/11 09:00
MHDA- 2009/06/09 09:00
CRDT- 2009/04/11 09:00
PHST- 2009/04/11 09:00 [entrez]
PHST- 2009/04/11 09:00 [pubmed]
PHST- 2009/06/09 09:00 [medline]
AID - JST.JSTAGE/analsci/25.553 [pii]
AID - 10.2116/analsci.25.553 [doi]
PST - ppublish
SO  - Anal Sci. 2009 Apr;25(4):553-7. doi: 10.2116/analsci.25.553.