PMID- 19363072
OWN - NLM
STAT- MEDLINE
DCOM- 20090615
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 75
IP  - 11
DP  - 2009 Jun
TI  - Differential utilization of carbon substrates by bacteria and fungi in tundra 
      soil.
PG  - 3611-20
LID - 10.1128/AEM.02865-08 [doi]
AB  - Little is known about the contribution of bacteria and fungi to decomposition of 
      different carbon compounds in arctic soils, which are an important carbon store 
      and possibly vulnerable to climate warming. Soil samples from a subarctic tundra 
      heath were incubated with (13)C-labeled glucose, acetic acid, glycine, starch, 
      and vanillin, and the incorporation of (13)C into different phospholipid fatty 
      acids (PLFA; indicative of growth) and neutral lipid fatty acids (NLFA; 
      indicative of fungal storage) was measured after 1 and 7 days. The use of 
      (13)C-labeled substrates allowed the addition of substrates at concentrations low 
      enough not to affect the total amount of PLFA. The label of glucose and acetic 
      acid was rapidly incorporated into the PLFA in a pattern largely corresponding to 
      the fatty acid concentration profile, while glycine and especially starch were 
      mainly taken up by bacteria and not fungi, showing that different groups of the 
      microbial community were responsible for substrate utilization. The 
      (13)C-incorporation from the complex substrates (starch and vanillin) increased 
      over time. There was significant allocation of (13)C into the fungal NLFA, except 
      for starch. For glucose, acetic acid, and glycine, the allocation decreased over 
      time, indicating use of the storage products, whereas for vanillin incorporation 
      into fungal NLFA increased during the incubation. In addition to providing 
      information on functioning of the microbial communities in an arctic soil, our 
      study showed that the combination of PLFA and NLFA analyses yields additional 
      information on the dynamics of substrate degradation.
FAU - Rinnan, Riikka
AU  - Rinnan R
AD  - Department of Microbial Ecology, Lund University, Sweden. riikkar@bio.ku.dk
FAU - Baath, Erland
AU  - Baath E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090410
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Carbon Isotopes)
RN  - 0 (Fatty Acids)
RN  - 0 (Phospholipids)
RN  - 7440-44-0 (Carbon)
RN  - 9005-25-8 (Starch)
RN  - IY9XDZ35W2 (Glucose)
RN  - Q40Q9N063P (Acetic Acid)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Acetic Acid/metabolism
MH  - Arctic Regions
MH  - Bacteria/*metabolism
MH  - Carbon/*metabolism
MH  - Carbon Isotopes/metabolism
MH  - Fatty Acids/metabolism
MH  - Fungi/*metabolism
MH  - Glucose/metabolism
MH  - Glycine/metabolism
MH  - Phospholipids/metabolism
MH  - *Soil Microbiology
MH  - Starch/metabolism
PMC - PMC2687315
EDAT- 2009/04/14 09:00
MHDA- 2009/06/16 09:00
PMCR- 2009/12/01
CRDT- 2009/04/14 09:00
PHST- 2009/04/14 09:00 [entrez]
PHST- 2009/04/14 09:00 [pubmed]
PHST- 2009/06/16 09:00 [medline]
PHST- 2009/12/01 00:00 [pmc-release]
AID - AEM.02865-08 [pii]
AID - 2865-08 [pii]
AID - 10.1128/AEM.02865-08 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2009 Jun;75(11):3611-20. doi: 10.1128/AEM.02865-08. Epub 
      2009 Apr 10.