PMID- 19363076
OWN - NLM
STAT- MEDLINE
DCOM- 20090615
LR  - 20240317
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 75
IP  - 11
DP  - 2009 Jun
TI  - Inter- and intraspecific variations of bacterial communities associated with 
      marine sponges from san juan island, washington.
PG  - 3513-21
LID - 10.1128/AEM.00002-09 [doi]
AB  - This study attempted to assess whether conspecific or congeneric sponges around 
      San Juan Island, Washington, harbor specific bacterial communities. We used a 
      combination of culture-independent DNA fingerprinting techniques (terminal 
      restriction fragment length polymorphism and denaturing gradient gel 
      electrophoresis [DGGE]) and culture-dependent approaches. The results indicated 
      that the bacterial communities in the water column consisted of more diverse 
      bacterial ribotypes than and were drastically different from those associated 
      with the sponges. High levels of similarity in sponge-associated bacterial 
      communities were found only in Myxilla incrustans and Haliclona rufescens, while 
      the bacterial communities in Halichondria panicea varied substantially among 
      sites. Certain terminal restriction fragments or DGGE bands were consistently 
      obtained for different individuals of M. incrustans and H. rufescens collected 
      from different sites, suggesting that there are stable or even specific 
      associations of certain bacteria in these two sponges. However, no specific 
      bacterial associations were found for H. panicea or for any one sponge genus. 
      Sequencing of nine DGGE bands resulted in recovery of seven sequences that best 
      matched the sequences of uncultured Proteobacteria. Three of these sequences fell 
      into the sponge-specific sequence clusters previously suggested. An uncultured 
      alphaproteobacterium and a culturable Bacillus sp. were found exclusively in all 
      M. incrustans sponges, while an uncultured gammaproteobacterium was unique to H. 
      rufescens. In contrast, the cultivation approach indicated that sponges contained 
      a large proportion of Firmicutes, especially Bacillus, and revealed large 
      variations in the culturable bacterial communities associated with congeneric and 
      conspecific sponges. This study revealed sponge species-specific but not genus- 
      or site-specific associations between sponges and bacterial communities and 
      emphasized the importance of using a combination of techniques for studying 
      microbial communities.
FAU - Lee, On On
AU  - Lee OO
AD  - Department of Biology, The Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong SAR, China.
FAU - Wong, Yue Him
AU  - Wong YH
FAU - Qian, Pei-Yuan
AU  - Qian PY
LA  - eng
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090410
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/genetics/*isolation & purification
MH  - *Biodiversity
MH  - Cluster Analysis
MH  - DNA Fingerprinting/methods
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porifera/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Washington
PMC - PMC2687283
EDAT- 2009/04/14 09:00
MHDA- 2009/06/16 09:00
PMCR- 2009/12/01
CRDT- 2009/04/14 09:00
PHST- 2009/04/14 09:00 [entrez]
PHST- 2009/04/14 09:00 [pubmed]
PHST- 2009/06/16 09:00 [medline]
PHST- 2009/12/01 00:00 [pmc-release]
AID - AEM.00002-09 [pii]
AID - 0002-09 [pii]
AID - 10.1128/AEM.00002-09 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2009 Jun;75(11):3513-21. doi: 10.1128/AEM.00002-09. Epub 
      2009 Apr 10.