PMID- 19363226
OWN - NLM
STAT- MEDLINE
DCOM- 20090526
LR  - 20190703
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 164
IP  - 15
DP  - 2009 Apr 11
TI  - Evaluation of unexpected positive results from a commercial ELISA for antibodies 
      to PRRSV.
PG  - 455-9
AB  - Unexpected positive results from the widely used IDEXX ELISA for the detection of 
      antibodies to porcine reproductive and respiratory syndrome virus (PRRSV) may 
      confound investigations of the disease. Supplementing the ELISA with blocking 
      agents and the use of IgG purified from serum samples had no effect on the 
      unexpected positive results, suggesting that they were due to an antibody-antigen 
      reaction. Simple competitive and blocking ELISAs were developed by modifying the 
      IDEXX ELISA, and they and an indirect fluorescent antibody test (IFAT) were used 
      to examine PRRSV antibodies in 33 antibody-negative, 88 antibody-positive and 73 
      unexpectedly positive sera. All the unexpectedly positive sera were negative by 
      IFAT, and 89.0 per cent were negative by both the competitive and blocking 
      ELISAs. The competitive ELISA (97.7 per cent) and the blocking ELISA (96.5 per 
      cent) detected more positive sera than the IFAT (90.9 per cent). These results 
      show that both ELISAs are capable of distinguishing positive and unexpectedly 
      positive sera, and suggest that most of the unexpected positive signals are 
      false-positives.
FAU - Okinaga, T
AU  - Okinaga T
AD  - Viral Diseases Research Team, National Institute of Animal Health, Kannondai, 
      Tsukuba, Ibaraki 305-0856, Japan.
FAU - Yamagishi, T
AU  - Yamagishi T
FAU - Yoshii, M
AU  - Yoshii M
FAU - Suzuki, T
AU  - Suzuki T
FAU - Miyazaki, A
AU  - Miyazaki A
FAU - Takagi, M
AU  - Takagi M
FAU - Tsunemitsu, H
AU  - Tsunemitsu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Enzyme-Linked Immunosorbent Assay/methods/standards/*veterinary
MH  - False Positive Reactions
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2009/04/14 09:00
MHDA- 2009/05/27 09:00
CRDT- 2009/04/14 09:00
PHST- 2009/04/14 09:00 [entrez]
PHST- 2009/04/14 09:00 [pubmed]
PHST- 2009/05/27 09:00 [medline]
AID - 164/15/455 [pii]
AID - 10.1136/vr.164.15.455 [doi]
PST - ppublish
SO  - Vet Rec. 2009 Apr 11;164(15):455-9. doi: 10.1136/vr.164.15.455.