PMID- 19364273
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20181201
IS  - 1937-3392 (Electronic)
IS  - 1937-3384 (Linking)
VI  - 16
IP  - 1
DP  - 2010 Feb
TI  - A novel method to isolate mesenchymal stem cells from bone marrow in a closed 
      system using a device made by nonwoven fabric.
PG  - 81-91
LID - 10.1089/ten.TEC.2008.0693 [doi]
AB  - Bone marrow stromal cells (BMSCs) include cells with multidirectional 
      differentiation potential described as mesenchymal stem cells. For clinical use, 
      it is important to develop a way to isolate BMSCs from bone marrow in a closed 
      system without centrifugation. After screening 200 biomaterials, we developed a 
      device containing a nonwoven fabric filter composed of rayon and polyethylene. 
      The filter selectively traps BMSCs among mononuclear cells in bone marrow based 
      on affinity, not cell size. The cells are then recovered by the retrograde flow. 
      Using canine and human bone marrow cells, the biological properties of BMSCs 
      isolated by the device were compared with those obtained by conventional methods 
      using centrifugation. The total number isolated by the device was larger, as was 
      the number of CD106(+)/STRO-1(+) double-positive cells. The cells showed 
      osteogenic, chondrogenic, and adipogenic differentiation potential in vitro. 
      Finally, the direct transplantation of cells isolated by the device without in 
      vitro cultivation accelerated bone regeneration in a canine model of 
      osteonecrosis in vivo. The proposed method is rapid and efficient, does not 
      require a biological clean area, and will be useful for the clinical application 
      of mesenchymal stem cells in bone marrow.
FAU - Ito, Kinya
AU  - Ito K
AD  - Institute for Frontier Medical Sciences, Kyoto University , Kyoto, Japan .
FAU - Aoyama, Tomoki
AU  - Aoyama T
FAU - Fukiage, Kenichi
AU  - Fukiage K
FAU - Otsuka, Seiji
AU  - Otsuka S
FAU - Furu, Moritoshi
AU  - Furu M
FAU - Jin, Yonghui
AU  - Jin Y
FAU - Nasu, Akira
AU  - Nasu A
FAU - Ueda, Michiko
AU  - Ueda M
FAU - Kasai, Yasunari
AU  - Kasai Y
FAU - Ashihara, Eishi
AU  - Ashihara E
FAU - Kimura, Shinya
AU  - Kimura S
FAU - Maekawa, Taira
AU  - Maekawa T
FAU - Kobayashi, Akira
AU  - Kobayashi A
FAU - Yoshida, Shinya
AU  - Yoshida S
FAU - Niwa, Hideo
AU  - Niwa H
FAU - Otsuka, Takanobu
AU  - Otsuka T
FAU - Nakamura, Takashi
AU  - Nakamura T
FAU - Toguchida, Junya
AU  - Toguchida J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part C Methods
JT  - Tissue engineering. Part C, Methods
JID - 101466663
RN  - 0 (Antigens, Surface)
RN  - 0 (Biocompatible Materials)
RN  - 0 (STRO-1 antigen, human)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 9002-88-4 (Polyethylene)
RN  - 9004-34-6 (Cellulose)
RN  - BX81F82EWG (rayon, purified)
SB  - IM
MH  - Adipocytes/cytology
MH  - Animals
MH  - Antigens, Surface/biosynthesis
MH  - Biocompatible Materials/*chemistry
MH  - Bone Marrow Cells/*cytology
MH  - Bone Regeneration
MH  - *Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cellulose/chemistry
MH  - Chondrocytes/cytology
MH  - Dogs
MH  - Humans
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microscopy, Electron, Scanning/methods
MH  - Osteonecrosis
MH  - Polyethylene/chemistry
MH  - Vascular Cell Adhesion Molecule-1/biosynthesis
EDAT- 2009/04/15 09:00
MHDA- 2010/06/09 06:00
CRDT- 2009/04/15 09:00
PHST- 2009/04/15 09:00 [entrez]
PHST- 2009/04/15 09:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
AID - 10.1089/ten.TEC.2008.0693 [doi]
PST - ppublish
SO  - Tissue Eng Part C Methods. 2010 Feb;16(1):81-91. doi: 10.1089/ten.TEC.2008.0693.