PMID- 19364494
OWN - NLM
STAT- MEDLINE
DCOM- 20090511
LR  - 20090504
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 383
IP  - 3
DP  - 2009 Jun 5
TI  - MCP-1 expressed by osteoclasts stimulates osteoclastogenesis in an 
      autocrine/paracrine manner.
PG  - 373-7
LID - 10.1016/j.bbrc.2009.04.020 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that plays a critical 
      role in the recruitment and activation of leukocytes. Here, we describe that 
      multinuclear osteoclast formation was significantly inhibited in cells derived 
      from MCP-1-deficient mice. MCP-1 has been implicated in the regulation of 
      osteoclast cell-cell fusion; however defects of multinuclear osteoclast formation 
      in the cells from mice deficient in DC-STAMP, a seven transmembrane receptor 
      essential for osteoclast cell-cell fusion, was not rescued by recombinant MCP-1. 
      The lack of MCP-1 in osteoclasts resulted in a down-regulation of DC-STAMP, 
      NFATc1, and cathepsin K, all of which were highly expressed in normal 
      osteoclasts, suggesting that osteoclast differentiation was inhibited in 
      MCP-1-deficient cells. MCP-1 alone did not induce osteoclastogenesis, however, 
      the inhibition of osteoclastogenesis in MCP-1-deficient cells was restored by 
      addition of recombinant MCP-1, indicating that osteoclastogenesis was regulated 
      in an autocrine/paracrine manner by MCP-1 under the stimulation of RANKL in 
      osteoclasts.
FAU - Miyamoto, Kana
AU  - Miyamoto K
AD  - Department of Orthopedic Surgery, Keio University School of Medicine, 
      Shinano-machi, Shinjuku-ku, Tokyo, Japan.
FAU - Ninomiya, Ken
AU  - Ninomiya K
FAU - Sonoda, Koh-Hei
AU  - Sonoda KH
FAU - Miyauchi, Yoshiteru
AU  - Miyauchi Y
FAU - Hoshi, Hiroko
AU  - Hoshi H
FAU - Iwasaki, Ryotaro
AU  - Iwasaki R
FAU - Miyamoto, Hiroya
AU  - Miyamoto H
FAU - Yoshida, Shigeyuki
AU  - Yoshida S
FAU - Sato, Yuiko
AU  - Sato Y
FAU - Morioka, Hideo
AU  - Morioka H
FAU - Chiba, Kazuhiro
AU  - Chiba K
FAU - Egashira, Kensuke
AU  - Egashira K
FAU - Suda, Toshio
AU  - Suda T
FAU - Toyama, Yoshiaki
AU  - Toyama Y
FAU - Miyamoto, Takeshi
AU  - Miyamoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090411
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DC-STAMP protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RANK Ligand)
RN  - 0 (Tnfsf11 protein, mouse)
SB  - IM
MH  - Animals
MH  - Autocrine Communication
MH  - *Cell Differentiation/genetics
MH  - Cell Fusion
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Tissue Proteins/metabolism
MH  - Osteoclasts/metabolism/*physiology
MH  - RANK Ligand/metabolism
EDAT- 2009/04/15 09:00
MHDA- 2009/05/12 09:00
CRDT- 2009/04/15 09:00
PHST- 2009/04/03 00:00 [received]
PHST- 2009/04/05 00:00 [accepted]
PHST- 2009/04/15 09:00 [entrez]
PHST- 2009/04/15 09:00 [pubmed]
PHST- 2009/05/12 09:00 [medline]
AID - S0006-291X(09)00701-3 [pii]
AID - 10.1016/j.bbrc.2009.04.020 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Jun 5;383(3):373-7. doi: 
      10.1016/j.bbrc.2009.04.020. Epub 2009 Apr 11.