PMID- 19365092
OWN - NLM
STAT- MEDLINE
DCOM- 20090831
LR  - 20211020
IS  - 0022-1554 (Print)
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Linking)
VI  - 57
IP  - 8
DP  - 2009 Aug
TI  - Apoptosis and desquamation of urothelial cells in tissue remodeling during rat 
      postnatal development.
PG  - 721-30
LID - 10.1369/jhc.2009.953349 [doi]
AB  - Postnatal rat urothelium was studied from day 0 to day 14, when intense cell loss 
      as part of tissue remodeling was expected. The morphological and biochemical 
      characteristics of urothelial cells in the tissue and released cells were 
      investigated by light and electron microscopy, by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay, by annexin V/propidium 
      iodide assay, and by immunofluorescent detection of active caspases and 
      tight-junction protein occludin. Intense apoptosis and massive desquamation were 
      detected between postnatal days 7 and 10. During this period, active caspases and 
      TUNEL-positive cells were found in the urothelium. Disassembled cell-cell 
      junctions were detected between cells. The majority of desquamated cells 
      expressed no apoptotic cell morphology, but were active caspase positive and 
      TUNEL positive. Ann+/PI- apoptotic bodies and desquamated Ann+/PI+ cells were 
      detected in the lumen. These results indicate that apoptosis and desquamation 
      participate in urothelial cell loss in the rat early postnatal period, 
      indispensable for fast urothelial remodeling during development.
FAU - Erman, Andreja
AU  - Erman A
AD  - Institute of Cell Biology, Faculty of Medicine, Lipiceva 2, 1000 Ljubljana, 
      Slovenia. andreja.erman@mf.uni-lj.si
FAU - Zupancic, Dasa
AU  - Zupancic D
FAU - Jezernik, Kristijan
AU  - Jezernik K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090413
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Membrane Proteins)
RN  - 0 (Occludin)
RN  - 0 (Ocln protein, rat)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *Apoptosis
MH  - Caspases/metabolism
MH  - Epithelial Cells/*cytology/ultrastructure
MH  - Female
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Occludin
MH  - Rats
MH  - Rats, Wistar
MH  - Tight Junctions/metabolism/ultrastructure
MH  - Urinary Bladder/cytology/growth & development/metabolism
MH  - Urothelium/*cytology/*growth & development/metabolism
PMC - PMC2713072
EDAT- 2009/04/15 09:00
MHDA- 2009/09/01 06:00
PMCR- 2009/08/01
CRDT- 2009/04/15 09:00
PHST- 2009/04/15 09:00 [entrez]
PHST- 2009/04/15 09:00 [pubmed]
PHST- 2009/09/01 06:00 [medline]
PHST- 2009/08/01 00:00 [pmc-release]
AID - jhc.2009.953349 [pii]
AID - 953349 [pii]
AID - 10.1369/jhc.2009.953349 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2009 Aug;57(8):721-30. doi: 10.1369/jhc.2009.953349. Epub 
      2009 Apr 13.