PMID- 19365690 OWN - NLM STAT- MEDLINE DCOM- 20091021 LR - 20231120 IS - 1438-7573 (Electronic) IS - 1525-3961 (Print) IS - 1438-7573 (Linking) VI - 10 IP - 3 DP - 2009 Sep TI - Enhanced survival of spiral ganglion cells after cessation of treatment with brain-derived neurotrophic factor in deafened guinea pigs. PG - 355-67 LID - 10.1007/s10162-009-0170-2 [doi] AB - Exogenous delivery of neurotrophic factors into the cochlea of deafened animals rescues spiral ganglion cells (SGCs) from degeneration. To be clinically relevant for human cochlear implant candidates, the protective effect of neurotrophins should persist after cessation of treatment and the treated SGCs should remain functional. In this study, the survival and functionality of SGCs were investigated after temporary treatment with brain-derived neurotrophic factor (BDNF). Guinea pigs in the experimental group were deafened, and 2 weeks later, the right cochleae were implanted with an electrode array and drug delivery cannula. BDNF was administered to the implanted cochleae during a 4-week period via a mini-osmotic pump. After completion of the treatment, the osmotic pumps were removed. Two weeks later, the animals were killed and the survival of SGCs was analyzed. To monitor the functionality of the auditory nerve, electrically evoked auditory brainstem responses (eABRs) were recorded in awake animals throughout the experiment. BDNF treatment resulted in enhanced survival of SGCs 2 weeks after cessation of the treatment and prevented the decreases in size and circularity that are seen in the untreated contralateral cochleae. The amplitude of the suprathreshold eABR response in BDNF-treated animals was significantly larger than in deafened control animals and comparable to that in normal-hearing control animals. The amplitude in the BDNF-treated group did not decrease significantly after cessation of treatment. The eABR latency in BDNF-treated animals was longer than normal and comparable to that in deafened control animals. These morphological and functional findings demonstrate that neurotrophic intervention had a lasting effect, which is promising for future clinical application of neurotrophic factors in implanted human cochleae. FAU - Agterberg, Martijn J H AU - Agterberg MJ AD - Department of Otorhinolaryngology, Rudolf Magnus Institute of Neuroscience, University Medical Center Utrecht, GA, Utrecht, The Netherlands. FAU - Versnel, Huib AU - Versnel H FAU - van Dijk, Lotte M AU - van Dijk LM FAU - de Groot, John C M J AU - de Groot JC FAU - Klis, Sjaak F L AU - Klis SF LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090414 PL - United States TA - J Assoc Res Otolaryngol JT - Journal of the Association for Research in Otolaryngology : JARO JID - 100892857 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Protein Synthesis Inhibitors) RN - 0 (Sodium Potassium Chloride Symporter Inhibitors) RN - 59-01-8 (Kanamycin) RN - 7LXU5N7ZO5 (Furosemide) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cell Survival/drug effects/physiology MH - Cochlear Implants MH - Deafness/chemically induced/*physiopathology MH - Electric Stimulation MH - Evoked Potentials, Auditory, Brain Stem/drug effects/physiology MH - Female MH - Furosemide/adverse effects MH - Guinea Pigs MH - Kanamycin/adverse effects MH - Models, Animal MH - Nerve Degeneration/*physiopathology/*prevention & control MH - Protein Synthesis Inhibitors/adverse effects MH - Sodium Potassium Chloride Symporter Inhibitors/adverse effects MH - Spiral Ganglion/*cytology/*drug effects/physiology PMC - PMC2717388 EDAT- 2009/04/15 09:00 MHDA- 2009/10/22 06:00 PMCR- 2009/04/14 CRDT- 2009/04/15 09:00 PHST- 2008/11/04 00:00 [received] PHST- 2009/03/20 00:00 [accepted] PHST- 2009/04/15 09:00 [entrez] PHST- 2009/04/15 09:00 [pubmed] PHST- 2009/10/22 06:00 [medline] PHST- 2009/04/14 00:00 [pmc-release] AID - 170 [pii] AID - 10.1007/s10162-009-0170-2 [doi] PST - ppublish SO - J Assoc Res Otolaryngol. 2009 Sep;10(3):355-67. doi: 10.1007/s10162-009-0170-2. Epub 2009 Apr 14.