PMID- 19365708
OWN - NLM
STAT- MEDLINE
DCOM- 20090909
LR  - 20211020
IS  - 1865-3774 (Electronic)
IS  - 0925-5710 (Linking)
VI  - 89
IP  - 5
DP  - 2009 Jun
TI  - Involvement of SRC-3 in deguelin-induced apoptosis in Jurkat cells.
PG  - 628-35
LID - 10.1007/s12185-009-0311-8 [doi]
AB  - The aim of the study was to investigate the anticancer effects and the molecular 
      mechanisms of deguelin on Jurkat cells. Cell viability was assessed by MTT assay. 
      Terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) 
      assay and transmission electron microscopy were used to detect cell apoptosis. A 
      propidium iodide method was used to study cell cycle distribution. RT-PCR and 
      Western blotting were employed to assess the expression levels of steroid 
      receptor coactivator-3 (SRC-3), nuclear factor-kappaB (NF-kappaB) and some 
      apoptosis related genes, including Bcl-2 and Bcl-xL. Deguelin was able to inhibit 
      cell proliferation by a cell-cycle arrest in the G(1)/G(0) phase and induce 
      apoptosis in Jurkat cells in vitro, with a 24-h IC(50) value of 43.73 +/- 0.35 
      nmol/L. The antileukemia effect of deguelin might be correlated well with the 
      downregulation of the expression of SRC-3 and its related transcription factor 
      NF-kappaB, which thus influenced the expression of apoptosis related genes Bcl-2 
      and Bcl-xL. Deguelin presented potent effects on growth arrest and apoptosis 
      induction in Jurkat cells in vitro via the interruption of SRC-3.
FAU - Li, Rui
AU  - Li R
AD  - Department of Hematology Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Chen, Yan
AU  - Chen Y
FAU - Shu, Wen-xiu
AU  - Shu WX
FAU - Chen, Zi
AU  - Chen Z
FAU - Ke, Wen-juan
AU  - Ke WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090414
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Trans-Activators)
RN  - 03L9OT429T (Rotenone)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA3 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 3)
RN  - K5Z93K66IE (deguelin)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Apoptosis Regulatory Proteins/genetics
MH  - Down-Regulation/drug effects/genetics
MH  - Histone Acetyltransferases/*physiology
MH  - Humans
MH  - Jurkat Cells
MH  - NF-kappa B/genetics
MH  - Nuclear Receptor Coactivator 3
MH  - Rotenone/*analogs & derivatives/pharmacology
MH  - Trans-Activators/*physiology
EDAT- 2009/04/15 09:00
MHDA- 2009/09/10 06:00
CRDT- 2009/04/15 09:00
PHST- 2008/10/08 00:00 [received]
PHST- 2009/03/24 00:00 [accepted]
PHST- 2009/02/22 00:00 [revised]
PHST- 2009/04/15 09:00 [entrez]
PHST- 2009/04/15 09:00 [pubmed]
PHST- 2009/09/10 06:00 [medline]
AID - 10.1007/s12185-009-0311-8 [doi]
PST - ppublish
SO  - Int J Hematol. 2009 Jun;89(5):628-35. doi: 10.1007/s12185-009-0311-8. Epub 2009 
      Apr 14.