PMID- 19366503
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20191210
IS  - 0003-7028 (Print)
IS  - 0003-7028 (Linking)
VI  - 63
IP  - 4
DP  - 2009 Apr
TI  - Targeted surface-enhanced Raman scattering nanosensors for whole-cell pH imagery.
PG  - 387-95
LID - 10.1366/000370209787944406 [doi]
AB  - In this paper the ability to develop a gold-core, silver-shell surface-enhanced 
      Raman scattering (SERS) nanosensor that has both a targeting component as well as 
      a sensing component is demonstrated. The nanosensor is capable of targeting 
      through the FcepsilonRI receptor-mediated endocytic pathway due to the adsorption 
      of 2,4-epsilon-dinitrophenol-L-lysine (DNP) ligand on the nanoparticle surface. 
      The nanosensor is also sensitive to pH changes in the endocytic vesicle within 
      the pH range of 4.5-7.5 by 4-mercaptopyridine (4-MPy) adsorbed to the particle 
      surface. The targeting and sensing moieties do not significantly interfere with 
      each other's function. The sensing component of the nanosensor is calibrated with 
      in vivo measurements of rat basophil leukemia (RBL-2H3) cells in standard buffer 
      solutions using the ionophore nigericin, which serves to equilibrate the external 
      [H(+)] concentration with that of the cell compartments. The targeting of the 
      nanosensor is verified with a beta-hexosaminidase assay. It is also demonstrated 
      that the targeted nanosensor is capable of making accurate cellular pH 
      measurements in RBL-2H3 cells, out to ninety minutes after targeted nanosensor 
      addition. Whole-cell, time-lapse, hyperspectral image cubes demonstrating 
      endocytic vesicular pH changes during FcepsilonRI receptor mediated endocytosis 
      are demonstrated.
FAU - Nowak-Lovato, K L
AU  - Nowak-Lovato KL
AD  - Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
FAU - Rector, K D
AU  - Rector KD
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Spectrosc
JT  - Applied spectroscopy
JID - 0372406
RN  - 0 (MS4A2 protein, human)
RN  - 0 (Pyridines)
RN  - 0 (Receptors, IgE)
RN  - 1094-76-4 (epsilon-dinitrophenyllysine)
RN  - 3M4G523W1G (Silver)
RN  - 4556-23-4 (4-thiopyridine)
RN  - 7440-57-5 (Gold)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Adsorption
MH  - Biosensing Techniques/*methods
MH  - Calibration
MH  - Cell Line, Tumor
MH  - Endocytosis
MH  - Gold/chemistry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lysine/*analogs & derivatives/chemistry/metabolism
MH  - Nanostructures/*chemistry
MH  - Pyridines/chemistry
MH  - Receptors, IgE/chemistry/*metabolism
MH  - Silver/chemistry
MH  - Spectrum Analysis, Raman/*methods
MH  - Surface Properties
EDAT- 2009/04/16 09:00
MHDA- 2009/05/08 09:00
CRDT- 2009/04/16 09:00
PHST- 2009/04/16 09:00 [entrez]
PHST- 2009/04/16 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
AID - 10.1366/000370209787944406 [doi]
PST - ppublish
SO  - Appl Spectrosc. 2009 Apr;63(4):387-95. doi: 10.1366/000370209787944406.