PMID- 19366663
OWN - NLM
STAT- MEDLINE
DCOM- 20090605
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 106
IP  - 17
DP  - 2009 Apr 28
TI  - Localized delivery of fibroblast growth factor-2 and brain-derived neurotrophic 
      factor reduces spontaneous seizures in an epilepsy model.
PG  - 7191-6
LID - 10.1073/pnas.0810710106 [doi]
AB  - A loss of neurons is observed in the hippocampus of many patients with epilepsies 
      of temporal lobe origin. It has been hypothesized that damage limitation or 
      repair, for example using neurotrophic factors (NTFs), may prevent the 
      transformation of a normal tissue into epileptic (epileptogenesis). Here, we used 
      viral vectors to locally supplement two NTFs, fibroblast growth factor-2 (FGF-2) 
      and brain-derived neurotrophic factor (BDNF), when epileptogenic damage was 
      already in place. These vectors were first characterized in vitro, where they 
      increased proliferation of neural progenitors and favored their differentiation 
      into neurons, and they were then tested in a model of status epilepticus-induced 
      neurodegeneration and epileptogenesis. When injected in a lesioned hippocampus, 
      FGF-2/BDNF expressing vectors increased neuronogenesis, embanked neuronal damage, 
      and reduced epileptogenesis. It is concluded that reduction of damage reduces 
      epileptogenesis and that supplementing specific NTFs in lesion areas represents a 
      new approach to the therapy of neuronal damage and of its consequences.
FAU - Paradiso, Beatrice
AU  - Paradiso B
AD  - Department of Clinical and Experimental Medicine, Section of Pharmacology, 
      University of Ferrara, 44100 Ferrara, Italy.
FAU - Marconi, Peggy
AU  - Marconi P
FAU - Zucchini, Silvia
AU  - Zucchini S
FAU - Berto, Elena
AU  - Berto E
FAU - Binaschi, Anna
AU  - Binaschi A
FAU - Bozac, Aleksandra
AU  - Bozac A
FAU - Buzzi, Andrea
AU  - Buzzi A
FAU - Mazzuferi, Manuela
AU  - Mazzuferi M
FAU - Magri, Eros
AU  - Magri E
FAU - Navarro Mora, Graciela
AU  - Navarro Mora G
FAU - Rodi, Donata
AU  - Rodi D
FAU - Su, Tao
AU  - Su T
FAU - Volpi, Ilaria
AU  - Volpi I
FAU - Zanetti, Lara
AU  - Zanetti L
FAU - Marzola, Andrea
AU  - Marzola A
FAU - Manservigi, Roberto
AU  - Manservigi R
FAU - Fabene, Paolo F
AU  - Fabene PF
FAU - Simonato, Michele
AU  - Simonato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090406
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Proliferation
MH  - Epilepsy/*genetics/metabolism/pathology/*therapy
MH  - Fibroblast Growth Factor 2/genetics/*metabolism
MH  - Genetic Therapy
MH  - Genetic Vectors/genetics
MH  - Male
MH  - Neurogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/*genetics/metabolism/pathology/*therapy
MH  - Treatment Outcome
PMC - PMC2678472
COIS- The authors declare no conflict of interest.
EDAT- 2009/04/16 09:00
MHDA- 2009/06/09 09:00
PMCR- 2009/10/28
CRDT- 2009/04/16 09:00
PHST- 2009/04/16 09:00 [entrez]
PHST- 2009/04/16 09:00 [pubmed]
PHST- 2009/06/09 09:00 [medline]
PHST- 2009/10/28 00:00 [pmc-release]
AID - 0810710106 [pii]
AID - 7574 [pii]
AID - 10.1073/pnas.0810710106 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7191-6. doi: 
      10.1073/pnas.0810710106. Epub 2009 Apr 6.