PMID- 19368777
OWN - NLM
STAT- MEDLINE
DCOM- 20090508
LR  - 20090416
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 27
IP  - 13
DP  - 2009 Mar 18
TI  - Immunogenicity of the highly pathogenic porcine reproductive and respiratory 
      syndrome virus GP5 protein encoded by a synthetic ORF5 gene.
PG  - 1957-63
LID - 10.1016/j.vaccine.2009.01.098 [doi]
AB  - Since May 2006, a highly pathogenic porcine reproductive and respiratory syndrome 
      virus (PRRSV), which causes continuous high fever and a high proportion of deaths 
      in vaccinated pigs of all ages, has emerged and prevailed in Mainland China. Huge 
      efforts should be made towards the development of an efficient vaccine against 
      the highly pathogenic PRRSV. Although the ORF5-encoded GP5 is the most important 
      immunogenic protein, accumulating evidences have demonstrated that incomplete 
      protection conferred by GP5-based vaccines. The inability to induce robust 
      protective immunity has been postulated to be associated with the presence of a 
      non-neutralizing decoy epitope and heavy glycosylation in close to its 
      neutralizing epitope. In this study, a synthetic ORF5 gene (SynORF5) was 
      engineered with the codon usage optimized for mammalian cell expression based on 
      the native ORF5 gene of highly pathogenic PRRSV strain WUH3. Additional 
      modifications, i.e., inserting a Pan DR T-helper cell epitope (PADRE) between the 
      neutralizing epitope and the non-neutralizing decoy epitope, and mutating four 
      potential N-glycosylation sites (N30, N34, N35 and N51) were also included in the 
      synthetic ORF5 gene. The immunogenicity of the SynORF5-encoded GP5 was evaluated 
      by DNA vaccination in mice and piglets. Results showed that significantly 
      enhanced GP5-specific ELISA antibody, PRRSV-specific neutralizing antibody, 
      IFN-gamma level, as well as lymphocyte proliferation response, could be induced 
      in mice and piglets immunized with DNA construct encoding the modified GP5 than 
      those received DNA vaccine expressing the native GP5. The enhanced immunogenicity 
      of the modified GP5 will be useful to facilitate the development of efficient 
      vaccines against the highly pathogenic PRRSV in the future.
FAU - Li, Bin
AU  - Li B
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, PR China.
FAU - Xiao, Shaobo
AU  - Xiao S
FAU - Wang, Yanwei
AU  - Wang Y
FAU - Xu, Shanshan
AU  - Xu S
FAU - Jiang, Yunbo
AU  - Jiang Y
FAU - Chen, Huanchun
AU  - Chen H
FAU - Fang, Liurong
AU  - Fang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090131
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Genes, Synthetic/*immunology
MH  - Genes, Viral/immunology
MH  - Glycosylation
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Swine/immunology/virology
MH  - Vaccines, DNA/immunology
MH  - Viral Envelope Proteins/*immunology
MH  - Viral Vaccines/*immunology
EDAT- 2009/04/17 09:00
MHDA- 2009/05/09 09:00
CRDT- 2009/04/17 09:00
PHST- 2008/10/10 00:00 [received]
PHST- 2009/01/12 00:00 [revised]
PHST- 2009/01/22 00:00 [accepted]
PHST- 2009/04/17 09:00 [entrez]
PHST- 2009/04/17 09:00 [pubmed]
PHST- 2009/05/09 09:00 [medline]
AID - S0264-410X(09)00161-3 [pii]
AID - 10.1016/j.vaccine.2009.01.098 [doi]
PST - ppublish
SO  - Vaccine. 2009 Mar 18;27(13):1957-63. doi: 10.1016/j.vaccine.2009.01.098. Epub 
      2009 Jan 31.