PMID- 19368844
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140729
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1260
DP  - 2009 Mar 13
TI  - Sex-specific 24-h profile of extracellular serotonin levels in the medial 
      prefrontal cortex.
PG  - 30-7
AB  - The medial prefrontal cortex (mPFC) controls emotional responses in many 
      species,receiving serotonergic innervation from the dorsal and median raphe 
      nucleus (DRN and MRN). To examine the sex difference in 24-h profiles of 
      extracellular serotonin (5HT) levels in the mPFC, an in vivo microdialysis study 
      was performed using intact male, diestrous female, and proestrous female rats. 
      Dialysates were automatically collected by a microdialysis probe from the mPFC 
      every 30 min for more than 24 h under freely moving conditions. The levels of 5HT 
      in dialysates were quantified by high performance liquid chromatography. 
      Extracellular 5HT levels exhibited episodic changes in the mPFC of both sexes of 
      rats, with both diestrous and proestrous females exhibiting a clear diurnal 
      change;the 5HT levels were high during the dark phase, but low during the light 
      phase. In contrast,male rats exhibited relatively high 5HT levels throughout the 
      day without significant diurnal changes. At mathematically analyzed trough, males 
      showed higher 5HT levels than diestrous or proestrous females. The overall 24-h 
      5HT levels in males were significantly greater than proestrous females, but were 
      not different from diestrous females. Further,stereological methods were used to 
      examine the number of tryptophan hydroxylase (TrpH),but no sex differences in the 
      number of TrpH immunoreactive cells in the DRN and MRN were observed. These 
      results suggest that sex and/or the gonadal steroid environment may affect the 
      24-h profile of extracellular 5HT in the mPFC of rats without changes in the 
      number of 5HT neurons in the DRN and MRN.
FAU - Jitsuki, Susumu
AU  - Jitsuki S
AD  - Department of Physiology, Yokohama City University Graduate School of Medicine, 
      3-9 Fukuura Kanazawa-ku, Yokohama 236-0004, Japan.
FAU - Kimura, Fukuko
AU  - Kimura F
FAU - Funabashi, Toshiya
AU  - Funabashi T
FAU - Takahashi, Takuya
AU  - Takahashi T
FAU - Mitsushima, Dai
AU  - Mitsushima D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090107
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 333DO1RDJY (Serotonin)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
SB  - IM
MH  - Animals
MH  - Cell Size
MH  - Chromatography, High Pressure Liquid
MH  - Diestrus/metabolism
MH  - Extracellular Space/*metabolism
MH  - Female
MH  - Male
MH  - Microdialysis
MH  - Organ Size
MH  - Periodicity
MH  - Photomicrography
MH  - Photoperiod
MH  - Prefrontal Cortex/anatomy & histology/*physiology
MH  - Proestrus/metabolism
MH  - Rats, Wistar
MH  - Serotonin/*metabolism
MH  - *Sex Characteristics
MH  - Time Factors
MH  - Tryptophan Hydroxylase/metabolism
EDAT- 2009/04/17 09:00
MHDA- 2015/03/31 06:00
CRDT- 2009/04/17 09:00
PHST- 2008/09/22 00:00 [received]
PHST- 2008/12/03 00:00 [revised]
PHST- 2008/12/23 00:00 [accepted]
PHST- 2009/04/17 09:00 [entrez]
PHST- 2009/04/17 09:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - S0006-8993(09)00128-0 [pii]
AID - 10.1016/j.brainres.2008.12.084 [doi]
PST - ppublish
SO  - Brain Res. 2009 Mar 13;1260:30-7. doi: 10.1016/j.brainres.2008.12.084. Epub 2009 
      Jan 7.