PMID- 19370085
OWN - NLM
STAT- MEDLINE
DCOM- 20090707
LR  - 20131121
IS  - 0008-4212 (Print)
IS  - 0008-4212 (Linking)
VI  - 87
IP  - 4
DP  - 2009 Apr
TI  - Comparative analysis of the bioenergetics of adult cardiomyocytes and nonbeating 
      HL-1 cells: respiratory chain activities, glycolytic enzyme profiles, and 
      metabolic fluxes.
PG  - 318-26
LID - 10.1139/Y09-018 [doi]
AB  - Comparative analysis of the bioenergetic parameters of adult rat cardiomyocytes 
      (CM) and HL-1 cells with very different structure but similar cardiac phenotype 
      was carried out with the aim of revealing the importance of the cell structure 
      for regulation of its energy fluxes. Confocal microscopic analysis showed very 
      different mitochondrial arrangement in these cells. The cytochrome content per 
      milligram of cell protein was decreased in HL-1 cells by a factor of 7 compared 
      with CM. In parallel, the respiratory chain complex activities were decreased by 
      4-8 times in the HL-1 cells. On the contrary, the activities of glycolytic 
      enzymes, hexokinase (HK), and pyruvate kinase (PK) were increased in HL-1 cells, 
      and these cells effectively transformed glucose into lactate. At the same time, 
      the creatine kinase (CK) activity was significantly decreased in HL-1 cells. In 
      conclusion, the results of this study comply with the assumption that in contrast 
      to CM in which oxidative phosphorylation is a predominant provider of ATP and the 
      CK system is a main carrier of energy from mitochondria to ATPases, in HL-1 cells 
      the energy metabolism is based mostly on the glycolytic reactions coupled to 
      oxidative phosphorylation through HK.
FAU - Monge, Claire
AU  - Monge C
AD  - INSERM U884, Laboratory of Fundamental and Applied Bioenergetics, Joseph Fourier 
      University, Grenoble, France.
FAU - Beraud, Nathalie
AU  - Beraud N
FAU - Tepp, Kersti
AU  - Tepp K
FAU - Pelloux, Sophie
AU  - Pelloux S
FAU - Chahboun, Siham
AU  - Chahboun S
FAU - Kaambre, Tuuli
AU  - Kaambre T
FAU - Kadaja, Lumme
AU  - Kadaja L
FAU - Roosimaa, Mart
AU  - Roosimaa M
FAU - Piirsoo, Andres
AU  - Piirsoo A
FAU - Tourneur, Yves
AU  - Tourneur Y
FAU - Kuznetsov, Andrey V
AU  - Kuznetsov AV
FAU - Saks, Valdur
AU  - Saks V
FAU - Seppet, Enn
AU  - Seppet E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Canada
TA  - Can J Physiol Pharmacol
JT  - Canadian journal of physiology and pharmacology
JID - 0372712
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Adenosine Diphosphate/metabolism
MH  - Animals
MH  - Cell Line
MH  - Creatine Kinase/metabolism
MH  - *Electron Transport
MH  - *Energy Metabolism
MH  - *Glycolysis
MH  - Hexokinase/metabolism
MH  - Mice
MH  - Myocytes, Cardiac/*metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2009/04/17 09:00
MHDA- 2009/07/08 09:00
CRDT- 2009/04/17 09:00
PHST- 2009/04/17 09:00 [entrez]
PHST- 2009/04/17 09:00 [pubmed]
PHST- 2009/07/08 09:00 [medline]
AID - y09-018 [pii]
AID - 10.1139/Y09-018 [doi]
PST - ppublish
SO  - Can J Physiol Pharmacol. 2009 Apr;87(4):318-26. doi: 10.1139/Y09-018.