PMID- 19371017
OWN - NLM
STAT- MEDLINE
DCOM- 20090625
LR  - 20131121
IS  - 0041-3771 (Print)
IS  - 0041-3771 (Linking)
VI  - 51
IP  - 2
DP  - 2009
TI  - [AO distribution and fluorescence spectra in myoblasts and single muscle fibres].
PG  - 103-10
AB  - Using spectral scanning regime of Leica TCS SL confocal microscope, acridine 
      orange (AO) fluorescence spectra in nuclei and cytoplasms of living myoblasts 
      L6J1 and frog single muscle fibres have been studied. AO fluorescence spectra in 
      salt solutions dependent on free AO concentrations and in AO complexes with DNA 
      have also been obtained for comparison. Myoblasts nuclei fluoresced in green 
      spectral region with maximum at approximately 530 nm (corresponding AO monomers 
      fluorescence), nucleoli fluoresced most brightly. Nuclear chromatin fluoresced 
      not uniformly in these cells. We saw similar to myoblasts AO emission in nucleoli 
      and nuclei of frog single muscle fibres. The uniformed weak green fluorescence 
      was observed for myoblast cytoplasm. As to the muscle fibres sarcoplasm, we saw 
      also AO green fluorescence in A-discs. In myoblasts and muscle fibre cytoplasm we 
      saw the fluorescent red, yellow and green granules which were acidic organelles. 
      The comparison of AO fluorescence spectra in living cells with fluorescence 
      spectra of different AO concentrations and complexes of AO with DNA in buffer 
      solutions allows estimation of AO concentration in acidic granules which is of 
      interest in the investigation of cellular organelles functions in the processes 
      of intracellular transport, adaptation, apoptosis and a number of pathological 
      conditions.
FAU - Beliaeva, T N
AU  - Beliaeva TN
FAU - Krolenko, S A
AU  - Krolenko SA
FAU - Leont'eva, E A
AU  - Leont'eva EA
FAU - Mozhenok, T P
AU  - Mozhenok TP
FAU - Salova, A V
AU  - Salova AV
FAU - Faddeeva, M D
AU  - Faddeeva MD
LA  - rus
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Russia (Federation)
TA  - Tsitologiia
JT  - Tsitologiia
JID - 0417363
RN  - 0 (Fluorescent Dyes)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/analysis/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleolus/metabolism
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - DNA/metabolism
MH  - Fluorescence
MH  - Fluorescent Dyes/analysis/*metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Muscle Fibers, Skeletal/chemistry/*metabolism
MH  - Myoblasts/chemistry/*metabolism
MH  - Organelles/metabolism
MH  - Rana temporaria
MH  - Rats
EDAT- 2009/04/18 09:00
MHDA- 2009/06/26 09:00
CRDT- 2009/04/18 09:00
PHST- 2009/04/18 09:00 [entrez]
PHST- 2009/04/18 09:00 [pubmed]
PHST- 2009/06/26 09:00 [medline]
PST - ppublish
SO  - Tsitologiia. 2009;51(2):103-10.