PMID- 19371451 OWN - NLM STAT- MEDLINE DCOM- 20090928 LR - 20220317 IS - 1469-8730 (Electronic) IS - 0967-1994 (Linking) VI - 17 IP - 3 DP - 2009 Aug TI - The effects of brain-derived neurotrophic factor and metformin on in vitro developmental competence of bovine oocytes. PG - 187-93 LID - 10.1017/S0967199409005255 [doi] AB - Brain-derived neurotrophic factor (BDNF) signalling via tyrosine kinase B receptors may play an important role in ovarian development and function. It has been reported that metformin elevates the activity of Tyrosine kinase receptors and may amplify BDNF signalling. The objective of this study was to investigate the effect of BDNF during in vitro maturation (IVM) and/or in vitro culture (IVC) (Experiment 1), and to evaluate the collaborative effect of BDNF and metformin treatment on the developmental competence of bovine in vitro fertilized (IVF) embryos (Experiment 2). In Experiment 1, BDNF, which was added to our previously established IVM systems, significantly increased the proportions of MII oocytes at both 10 ng/ml (86.7%) and 100 ng/ml (85.4%) compared with the control (64.0%). However, there was no statistically significant difference in blastocyst development between the control or BDNF-supplemented groups. In Experiment 2, in order to investigate the effect of BDNF (10 ng/ml) and/or metformin (10(-5) M) per se, TCM-199 without serum and hormones was used as the control IVM medium. The BDNF (48.3%) and BDNF plus metformin (56.5%) significantly enhanced the proportions of MII oocytes compared with the control (34.4%). Although, BDNF or metformin alone had no effect in embryo development, BDNF plus metformin significantly improved early embryo development to the 8-16-cell stage compared with the control (16.5 vs. 5.5%). In conclusion, the combination of BDNF and metformin may have a collaborative effect during the IVM period. These results could further contribute to the establishment of a more efficient bovine in vitro embryo production system. FAU - Hong, So Gun AU - Hong SG AD - Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. FAU - Jang, Goo AU - Jang G FAU - Oh, Hyun Ju AU - Oh HJ FAU - Koo, Ok Jae AU - Koo OJ FAU - Park, Jung Eun AU - Park JE FAU - Park, Hee Jung AU - Park HJ FAU - Kang, Sung Keun AU - Kang SK FAU - Lee, Byeong Chun AU - Lee BC LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090417 PL - England TA - Zygote JT - Zygote (Cambridge, England) JID - 9309124 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Recombinant Proteins) RN - 9100L32L2N (Metformin) RN - GAN16C9B8O (Glutathione) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cattle MH - Cumulus Cells/physiology MH - Embryo Culture Techniques MH - Embryonic Development/*drug effects/physiology MH - Fertilization in Vitro MH - Glutathione/analysis MH - Metaphase/*drug effects/physiology MH - Metformin/*pharmacology MH - Oocytes/*drug effects/physiology MH - Recombinant Proteins/pharmacology EDAT- 2009/04/18 09:00 MHDA- 2009/09/29 06:00 CRDT- 2009/04/18 09:00 PHST- 2009/04/18 09:00 [entrez] PHST- 2009/04/18 09:00 [pubmed] PHST- 2009/09/29 06:00 [medline] AID - S0967199409005255 [pii] AID - 10.1017/S0967199409005255 [doi] PST - ppublish SO - Zygote. 2009 Aug;17(3):187-93. doi: 10.1017/S0967199409005255. Epub 2009 Apr 17.