PMID- 19372669
OWN - NLM
STAT- MEDLINE
DCOM- 20090519
LR  - 20090417
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 124
IP  - 1
DP  - 2009
TI  - Chromosomal study of native and hatchery trouts from Italy (Salmo trutta complex, 
      Salmonidae): conventional and FISH analysis.
PG  - 51-62
LID - 10.1159/000200088 [doi]
AB  - A cytogenetic analysis was carried out using conventional staining, banding 
      techniques and fluorescence in situ hybridization (FISH) in Italian populations 
      of brown trout (Salmo truttacomplex). All individuals analysed, belonging to the 
      Atlantic (At), Marmoratus (Ma), Adriatic (Ad) and Mediterranean (Me) lineages, 
      showed remarkable karyotype uniformity, with diploid complement of 2n = 80 
      chromosomes, arm number (NF) of 102 and invariable karyotype composition. Such 
      uniformity was also observed with respect to the location of 5S rDNA and the 
      active, i.e. silver-positive NOR sites. On the contrary, FISH with 28S ribosomal 
      probe and fluorescent staining with CMA3 revealed that inactive NOR sites are 
      more numerous in Ad and Me than in At and Ma lineages. A centromeric sequence was 
      successfully isolated from Salmo trutta individuals by polymerase chain reaction 
      (PCR)-based cloning, using primers designed from published Atlantic salmon (Salmo 
      salar) satellite DNA sequences. This sequence had high AT content (65.3%) and 
      short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellite 
      repeats. The isolated satellite DNA clones were localized with FISH in the 
      centromeric regions of the brown trout chromosomes, showing lineage-specific 
      patterns. Because it is well known that AT-rich sequences can induce a pronounced 
      DNA curvature, which in turn would promote faster and higher chromatin 
      spiralization, it may be hypothesised that the wide distribution of this 
      satellite in the S. trutta genome may have played a role in its karyotype 
      stability. The presence of this sequence in other salmonid species was also 
      tested by Southern blot hybridization and used to analyze its evolution within 
      salmonids.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Caputo, V
AU  - Caputo V
AD  - Istituto di Biologia e Genetica, Universita Politecnica delle Marche, Ancona, 
      Italy. v.caputo@univpm.it
FAU - Giovannotti, M
AU  - Giovannotti M
FAU - Nisi Cerioni, P
AU  - Nisi Cerioni P
FAU - Splendiani, A
AU  - Splendiani A
FAU - Olmo, E
AU  - Olmo E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090415
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Satellite)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Alu Elements/genetics
MH  - Animals
MH  - Atlantic Ocean
MH  - Base Sequence
MH  - Chromosome Banding
MH  - Chromosomes/*genetics
MH  - Consensus Sequence
MH  - DNA Primers/chemistry
MH  - DNA, Ribosomal/genetics
MH  - DNA, Satellite/chemistry
MH  - Diploidy
MH  - *Fisheries
MH  - Fluorescent Dyes/metabolism
MH  - *In Situ Hybridization, Fluorescence
MH  - Italy
MH  - Karyotyping
MH  - Mediterranean Sea
MH  - Metaphase
MH  - Molecular Sequence Data
MH  - Nucleolus Organizer Region/metabolism
MH  - Oncorhynchus mykiss/*genetics
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - Salmo salar/*genetics
MH  - Silver Staining
MH  - Species Specificity
EDAT- 2009/04/18 09:00
MHDA- 2009/05/20 09:00
CRDT- 2009/04/18 09:00
PHST- 2008/09/03 00:00 [accepted]
PHST- 2009/04/18 09:00 [entrez]
PHST- 2009/04/18 09:00 [pubmed]
PHST- 2009/05/20 09:00 [medline]
AID - 000200088 [pii]
AID - 10.1159/000200088 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2009;124(1):51-62. doi: 10.1159/000200088. Epub 2009 Apr 
      15.