PMID- 19377825
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 61
IP  - 2
DP  - 2009 Apr 25
TI  - B16-F10 melanoma cells and cell culture supernatant enhance angiogenesis in mouse 
      ischemic limb.
PG  - 139-45
AB  - Generation of therapeutic angiogenesis to enhance vascularization in the ischemic 
      tissues is a method for treating ischemic tissues in atherosclerotic 
      cardiovascular artery disease. The chemokine stromal cell-derived factor-1 
      (SDF-1) and its receptor (CXC chemokine receptor 4, CXCR4) play a critical role 
      in the process of post-natal neovascularization. The SDF-1-CXCR4 axis is a 
      potential mechanism for the treatment of ischemic limb. Here, we investigated the 
      role of CXCR4 in bone marrow cells (BMCs) in neovascularization induced by tumor 
      cells and the supernatant of culture media in a murine hind-limb ischemia model 
      which was made by resecting femoral artery and vein. After the injection of mouse 
      melanoma cells B16-F10 (1x10(6) cells in 0.1 mL at the operation day, s.c.) into 
      the abdomen or the cell culture supernatant (0.1 mL/d for 21 d after operation, 
      i.m.) into the ischemic abductor muscle, the CXCR4 positive BMCs were analyzed by 
      flow cytometry. The perfusion of the ischemic limb was evaluated by laser Doppler 
      perfusion imaging (LDPI) on 7, 14 and 21 d after vascular injury operation. 
      Capillary endothelial alkaline phosphatase (AP) was stained to quantify the 
      presence of capillaries, and histological method was used to evaluate the 
      capillary density as a measure of neovascularization in ischemic tissues. The 
      proportions of CXCR4 positive BMCs were notably higher in ischemic limb injected 
      with tumor cells or the supernatant compared to those in the control group 
      (P<0.05). Injection of tumor cells or the supernatant resulted in significantly 
      improved perfusion as measured by LDPI perfusion ratios on 7, 14 and 21 d after 
      femoral artery and vein resection in mice, compared to the controls (P<0.05). 
      Tissue samples harvested from the lower calf muscle at day 21 demonstrated 
      increased capillary densities in mice receiving tumor cells (0.81+/-0.13) or the 
      supernatant (0.63+/-0.05), compared with those in control group (0.44+/-0.09, 
      P<0.05). In conclusion, the injection of B16-F10 tumor cells or the supernatant 
      induces the increase of CXCR4 positive cells in BMCs and the improvement of in 
      vivo neovasculogenesis in mouse ischemic limb.
FAU - Zhou, Tao
AU  - Zhou T
AD  - Department of Vascular Surgery, Fudan University, Shanghai, China.
FAU - Hu, Zhao-Hui
AU  - Hu ZH
FAU - Zhou, Bo
AU  - Zhou B
FAU - Fu, Wei-Guo
AU  - Fu WG
FAU - Wang, Yu-Qi
AU  - Wang YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (CXCR4 protein, mouse)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - Capillaries
MH  - Cell Culture Techniques
MH  - Chemokine CXCL12/metabolism
MH  - Culture Media, Conditioned/*pharmacology
MH  - Disease Models, Animal
MH  - Endothelium, Vascular
MH  - Hindlimb/*blood supply
MH  - Ischemia/*physiopathology
MH  - Melanoma, Experimental
MH  - Mice
MH  - Muscle, Skeletal/blood supply
MH  - *Neovascularization, Physiologic
MH  - Receptors, CXCR4/*metabolism
EDAT- 2009/04/21 09:00
MHDA- 2015/05/20 06:00
CRDT- 2009/04/21 09:00
PHST- 2009/04/21 09:00 [entrez]
PHST- 2009/04/21 09:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2009 Apr 25;61(2):139-45.