PMID- 19379153
OWN - NLM
STAT- MEDLINE
DCOM- 20090716
LR  - 20090421
IS  - 1744-9987 (Electronic)
IS  - 1744-9979 (Linking)
VI  - 13
IP  - 2
DP  - 2009 Apr
TI  - Granulocyte and monocyte adsorption apheresis therapy modulates monocyte-derived 
      dendritic cell function in patients with ulcerative colitis.
PG  - 138-46
LID - 10.1111/j.1744-9987.2009.00668.x [doi]
AB  - The aim of this study was to elucidate the molecular mechanisms responsible for 
      the therapeutic effects of granulocyte and monocyte adsorption apheresis (GMA). 
      We investigated the alterations in circulating monocyte subsets and 
      monocyte-derived dendritic cell (moDC) function after GMA therapy in ulcerative 
      colitis (UC) patients. Eighteen patients with UC were enrolled: 14 patients were 
      responders, and 4 patients were non-responders. Peripheral venous blood was 
      obtained within 5 min before and 5 min after GMA therapy. Flow cytometric 
      analysis for monocyte markers (CD14/CD16) was then performed. Monocyte-derived 
      dendritic cells were obtained and alterations in their phenotype were analyzed by 
      flow cytometry. Their function was also analyzed in a mixed lymphocyte reaction 
      assay between allo-naive T lymphocytes. Flow cytometric analysis for 
      intracellular interferon (IFN)-gamma (T-helper 1 cells) and interleukin (IL)-4 
      (T-helper 2 cells) was then performed for the stimulated T lymphocytes. In 
      patients who responded to GMA, the average numbers of monocytes, especially 
      CD16(+) monocytes, were significantly decreased after therapy (P < 0.05). In 
      responders, post-GMA moDCs expressed significantly lower CD80 and B7-DC, which 
      are one of the stimulation and maturation markers of dendritic cells, compared to 
      pre-GMA moDCs. CD83, CD86 and human leukocyte antigen-DR also showed a tendency 
      to decrease. In responders, naive T lymphocytes stimulated with post-GMA moDCs 
      produced significantly less IFN-gamma and IL-4 compared to those stimulated with 
      pre-GMA moDCs. The results of our study show that some of the immunosuppressive 
      effects of GMA therapy may be associated with the modulation of monocyte subsets 
      and moDC function.
FAU - Iwakami, Yuko
AU  - Iwakami Y
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine, 
      Keio University School of Medicine, Tokyo, Japan.
FAU - Sakuraba, Atsushi
AU  - Sakuraba A
FAU - Sato, Toshiro
AU  - Sato T
FAU - Takada, Yasuhiro
AU  - Takada Y
FAU - Izumiya, Motoko
AU  - Izumiya M
FAU - Ichikawa, Hitoshi
AU  - Ichikawa H
FAU - Hibi, Toshifumi
AU  - Hibi T
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - Australia
TA  - Ther Apher Dial
JT  - Therapeutic apheresis and dialysis : official peer-reviewed journal of the 
      International Society for Apheresis, the Japanese Society for Apheresis, the 
      Japanese Society for Dialysis Therapy
JID - 101181252
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Component Removal/*methods
MH  - Colitis, Ulcerative/blood/*therapy
MH  - Dendritic Cells/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Granulocytes/*metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-4/metabolism
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Phenotype
MH  - T-Lymphocytes/metabolism
MH  - Treatment Outcome
MH  - Young Adult
EDAT- 2009/04/22 09:00
MHDA- 2009/07/17 09:00
CRDT- 2009/04/22 09:00
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2009/07/17 09:00 [medline]
AID - TAP668 [pii]
AID - 10.1111/j.1744-9987.2009.00668.x [doi]
PST - ppublish
SO  - Ther Apher Dial. 2009 Apr;13(2):138-46. doi: 10.1111/j.1744-9987.2009.00668.x.