PMID- 1937942
OWN - NLM
STAT- MEDLINE
DCOM- 19911127
LR  - 20171206
IS  - 0391-3988 (Print)
IS  - 0391-3988 (Linking)
VI  - 14
IP  - 8
DP  - 1991 Aug
TI  - Cellular mechanisms of artificial peptides binding to HLA.
PG  - 518-22
AB  - On the basis of the consideration that cell-free models cannot precisely mimic 
      the complexity of the intracellular environment, we used a system to investigate 
      the mechanisms that enable antigen-presenting cells (APC) to bind exogenous 
      peptides through their human leukocyte antigen (HLA) molecules. We evaluated the 
      uptake of the radiolabeled peptide 17-29-Tyr of influenza virus matrix protein by 
      B-EBV cell lines, under various conditions. The results can be summarized as 
      follows: a) the kinetics of peptide binding and release are very fast in living, 
      fully competent cells; b) the peptide-HLA complexes are short-living and the DR 
      molecules continuously undergo peptidic exchange; c) using glutaraldehyde-fixed 
      cells, the kinetics of the two phenomena are slow, closely resembling those 
      observed with the same peptide and purified, immobilized DR molecules. The data 
      suggest that in APC, cellular mechanisms are operative that increase the 
      efficiency of both loading and unloading of Class II HLA with exogenous peptides. 
      This is likely to be related to the recycling of Class II molecules to 
      intracellular compartments, were binding takes place. The observation that the 
      HLA-peptide complex is a dynamic structure, suggests the possibility of replacing 
      natural peptides with synthetic ones at this level, in order to regulate the 
      immune response.
FAU - Frumento, G
AU  - Frumento G
AD  - Immunogenetic Department, National Institute for Cancer Research, Genova, Italy.
FAU - Pernis, B
AU  - Pernis B
FAU - Fais, F
AU  - Fais F
FAU - Vitale, M
AU  - Vitale M
FAU - Chersi, A
AU  - Chersi A
FAU - Ferrara, G B
AU  - Ferrara GB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Antigen-Presenting Cells/*metabolism
MH  - HLA Antigens/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Protein Binding
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PST - ppublish
SO  - Int J Artif Organs. 1991 Aug;14(8):518-22.