PMID- 19379593
OWN - NLM
STAT- MEDLINE
DCOM- 20110712
LR  - 20190816
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 17
IP  - 2
DP  - 2009 Apr
TI  - [Effects of TNF-alpha on MCP-1 and IL-8 mRNA expression in human umbilical vein 
      endothelial cells].
PG  - 479-82
AB  - This study was aimed to explore monocyte chemotactic protein 1 (MCP-1) and 
      interleukin 8 (IL-8) expressions in human umbilical vein endothelial cells 
      (HUVECs) affected by TNF-alpha and its molecular mechanism. RT-PCR assay was used 
      to detect the expression of MCP-1 and IL-8 mRNA in HUVECs at various times 
      following stimulation with TNF-alpha; the nuclear factor-kappa B (NF-kappaB) 
      activation in HUVECs were detected by immunofluorescence. The results showed that 
      after stimulation with NF-kappaB, the expression levels of MCP-1 and IL-8 mRNA in 
      HUVECs increased, and changed along with different time; the expression of IL-8 
      mRNA reached to peak level at 8 hours and the expression of MCP-1 mRNA reached to 
      peak level at 12 hours; the expression of NF-kappaB p65 protein began increasing 
      at 30 minutes and reached to peak level at 1 hour; then the staining of cytoplasm 
      gradually weakened, while staining of nuclei was enhanced, which indicated 
      significant nuclear translocation of NF-kappaB p65. It is concluded that the 
      TNF-alpha induces expressions of IL-8 mRNA, MCP-1 mRNA and NF-kappaB in HUVECs, 
      and NF-kappaB activities signal pathway may play a role in IL-8 mRNA and MCP-1 
      mRNA expressions.
FAU - Cheng, Hai-Tao
AU  - Cheng HT
AD  - Department of Cardiology, The Forth Hospital, Jilin University, Changchun 130011, 
      Jilin Province, China.
FAU - Yang, Tong
AU  - Yang T
FAU - Zhao, Jun
AU  - Zhao J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Endothelial Cells/*drug effects/metabolism
MH  - Endothelium, Vascular/cytology/drug effects/metabolism
MH  - Humans
MH  - Interleukin-8/*metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Umbilical Veins/cytology/metabolism
EDAT- 2009/04/22 09:00
MHDA- 2011/07/13 06:00
CRDT- 2009/04/22 09:00
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2011/07/13 06:00 [medline]
AID - 1009-2137(2009)02-0479-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Apr;17(2):479-82.