PMID- 19379664
OWN - NLM
STAT- MEDLINE
DCOM- 20091106
LR  - 20220331
IS  - 0928-4249 (Print)
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Linking)
VI  - 40
IP  - 4
DP  - 2009 Jul-Aug
TI  - Evidence of long distance airborne transport of porcine reproductive and 
      respiratory syndrome virus and Mycoplasma hyopneumoniae.
PG  - 39
LID - 10.1051/vetres/2009022 [doi]
LID - 39
AB  - The ability of porcine reproductive and respiratory syndrome virus (PRRSV) and 
      Mycoplasma hyopneumoniae to be transported over long distances via the airborne 
      route was evaluated. A source population of 300 grow-finish pigs was 
      experimentally inoculated with PRRSV MN-184 and M. hyopneumoniae 232 and over a 
      50-day period, air samples were collected at designated distances from the source 
      herd using a liquid cyclonic collector. Samples were tested for the presence of 
      PRRSV RNA and M. hyopneumoniae DNA by PCR and if positive, further characterized. 
      Of the 306 samples collected, 4 (1.3%) were positive for PRRSV RNA and 6 (1.9%) 
      were positive for M. hyopneumoniae DNA. The PRRSV-positive samples were recovered 
      4.7 km to the northwest (NW) of the source population. Four of the M. 
      hyopneumoniae-positive samples were obtained at the NW sampling point; 2 samples 
      at approximately 2.3 km and the other 2 samples approximately 4.7 km from the 
      source population. Of the remaining 2 samples, one sample was obtained at the 
      southeast sampling point and the other at the southwest sampling point, with both 
      locations being approximately 4.7 km from the source. The four PRRSV-positive 
      samples contained infectious virus and were >/= 98.8% homologous to the MN-184 
      isolate used to inoculate the source population. All 6 of the M. 
      hyopneumoniae-positive samples were 99.9% homologous to M. hyopneumoniae 232. 
      These results support the hypothesis that long distance airborne transport of 
      these important swine pathogens can occur.
FAU - Dee, Scott
AU  - Dee S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, St. Paul, MN 55108, USA. deexx004@umn.edu
FAU - Otake, Satoshi
AU  - Otake S
FAU - Oliveira, Simone
AU  - Oliveira S
FAU - Deen, John
AU  - Deen J
LA  - eng
PT  - Journal Article
DEP - 20090421
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
SB  - IM
MH  - *Air Microbiology
MH  - Animals
MH  - Mycoplasma hyopneumoniae/*physiology
MH  - Pneumonia of Swine, Mycoplasmal/*microbiology/transmission
MH  - Porcine Reproductive and Respiratory Syndrome/transmission/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - Wind
PMC - PMC2701181
EDAT- 2009/04/22 09:00
MHDA- 2009/11/07 06:00
PMCR- 2009/04/21
CRDT- 2009/04/22 09:00
PHST- 2009/01/27 00:00 [received]
PHST- 2009/04/17 00:00 [accepted]
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2009/11/07 06:00 [medline]
PHST- 2009/04/21 00:00 [pmc-release]
AID - v09268 [pii]
AID - 10.1051/vetres/2009022 [doi]
PST - ppublish
SO  - Vet Res. 2009 Jul-Aug;40(4):39. doi: 10.1051/vetres/2009022. Epub 2009 Apr 21.