PMID- 19380026
OWN - NLM
STAT- MEDLINE
DCOM- 20090625
LR  - 20220408
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 190
IP  - 2
DP  - 2009 Apr 15
TI  - Allelic status of 1p and 19q in oligodendrogliomas and glioblastomas: multiplex 
      ligation-dependent probe amplification versus loss of heterozygosity.
PG  - 93-6
LID - 10.1016/j.cancergencyto.2008.09.017 [doi]
AB  - Identification of the 1p/19q allelic status in gliomas, primarily those with a 
      major oligodendroglial component, has become an excellent molecular complement to 
      tumor histology in order to identify those cases sensitive to chemotherapy. In 
      addition to loss of heterozygosity (LOH), fluorescence in situ hybridization 
      (FISH), or comparative genomic hybridization (CGH), multiplex ligation-dependent 
      probe amplification (MLPA) has been shown to be an alternative methodology to 
      identify deletions of those chromosome arms. We used MLPA to explore the 1p and 
      19q allelic constitution in a series of 76 gliomas: 41 tumors with a major 
      oligodendroglial component, 34 glioblastomas, and one low-grade astrocytoma. We 
      compared the MLPA findings of the oligodendroglial cases with those previously 
      obtained using LOH in the same samples. Thirty-eight of 41 oligodendrogliomas 
      displayed identical findings by both LOH and MLPA, and losses at either 1p and/or 
      19q were identified in 12 of 35 (34%) astrocytic tumors. These findings agree 
      with data previously reported comparing MLPA versus FISH or CGH in gliomas and 
      suggest that MLPA can be used in the identification of the 1p/19q allelic 
      deletions on these brain neoplasms.
FAU - Franco-Hernandez, Carmen
AU  - Franco-Hernandez C
AD  - Research Unit-Unidad de Investigacion, Hospital Universiatrio La Paz, Paseo 
      Castellana 261, 28046 Madrid, Spain.
FAU - Martinez-Glez, Victor
AU  - Martinez-Glez V
FAU - de Campos, Jose M
AU  - de Campos JM
FAU - Isla, Alberto
AU  - Isla A
FAU - Vaquero, Jesus
AU  - Vaquero J
FAU - Gutierrez, Manuel
AU  - Gutierrez M
FAU - Casartelli, Cacilda
AU  - Casartelli C
FAU - Rey, Juan A
AU  - Rey JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Alleles
MH  - Brain Neoplasms/*genetics/pathology
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Glioblastoma/*genetics/pathology
MH  - Humans
MH  - Loss of Heterozygosity/*genetics
MH  - Nucleic Acid Amplification Techniques/methods
MH  - Oligodendroglioma/*genetics
EDAT- 2009/04/22 09:00
MHDA- 2009/06/26 09:00
CRDT- 2009/04/22 09:00
PHST- 2008/07/14 00:00 [received]
PHST- 2008/09/16 00:00 [revised]
PHST- 2008/09/26 00:00 [accepted]
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2009/06/26 09:00 [medline]
AID - S0165-4608(09)00009-0 [pii]
AID - 10.1016/j.cancergencyto.2008.09.017 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2009 Apr 15;190(2):93-6. doi: 
      10.1016/j.cancergencyto.2008.09.017.