PMID- 19381064
OWN - NLM
STAT- MEDLINE
DCOM- 20091209
LR  - 20211020
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 41
IP  - 8
DP  - 2009 Aug 31
TI  - Neuronal activation increases the density of eukaryotic translation initiation 
      factor 4E mRNA clusters in dendrites of cultured hippocampal neurons.
PG  - 601-10
LID - 10.3858/emm.2009.41.8.066 [doi]
AB  - Activity-dependent dendritic translation in CNS neurons is important for the 
      synapse-specific provision of proteins that may be necessary for strengthening of 
      synaptic connections. A major rate-limiting factor during protein synthesis is 
      the availability of eukaryotic translation initiation factor 4E (eIF4E), an mRNA 
      5-cap-binding protein. In this study we show by fluorescence in situ 
      hybridization (FISH) that the mRNA for eIF4E is present in the dendrites of 
      cultured rat hippocampal neurons. Under basal culture conditions, 58.7 +/-11.6% 
      of the eIF4E mRNA clusters localize with or immediately adjacent to PSD-95 
      clusters. Neuronal activation with KCl (60 mM, 10 min) very significantly 
      increases the number of eIF4E mRNA clusters in dendrites by 50.1 and 74.5% at 2 
      and 6 h after treatment, respectively. In addition, the proportion of eIF4E mRNA 
      clusters that localize with PSD-95 increases to 74.4+/-7.7% and 77.8+/-7.6% of 
      the eIF4E clusters at 2 and 6 h after KCl treatment, respectively. Our results 
      demonstrate the presence of eIF4E mRNA in dendrites and an activity-dependent 
      increase of these clusters at synaptic sites. This provides a potential mechanism 
      by which protein translation at synapses may be enhanced in response to synaptic 
      stimulation.
FAU - Moon, Il Soo
AU  - Moon IS
AD  - Department of Anatomy, College of Medicine, Dongguk University, Gyeongju 780-714, 
      Korea.
FAU - Cho, Sun-Jung
AU  - Cho SJ
FAU - Seog, Dae-Hyun
AU  - Seog DH
FAU - Walikonis, Randall
AU  - Walikonis R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (RNA, Messenger)
RN  - 660YQ98I10 (Potassium Chloride)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendrites/*metabolism
MH  - Eukaryotic Initiation Factor-4E/genetics/*metabolism
MH  - Hippocampus/*cytology
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Neurons/cytology/*physiology
MH  - Potassium Chloride/pharmacology
MH  - Protein Biosynthesis
MH  - RNA, Messenger/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synapses
MH  - *Up-Regulation
PMC - PMC2739900
EDAT- 2009/04/22 09:00
MHDA- 2009/12/16 06:00
PMCR- 2009/08/31
CRDT- 2009/04/22 09:00
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2009/08/31 00:00 [pmc-release]
AID - 10.3858/emm.2009.41.8.066 [doi]
PST - ppublish
SO  - Exp Mol Med. 2009 Aug 31;41(8):601-10. doi: 10.3858/emm.2009.41.8.066.