PMID- 19381397
OWN - NLM
STAT- MEDLINE
DCOM- 20100304
LR  - 20090421
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 134
IP  - 5
DP  - 2009 May
TI  - Novel fluorescence enhancement IgE assay using a DNA aptamer.
PG  - 1003-7
LID - 10.1039/b812450g [doi]
AB  - In this paper, we demonstrate a fluorescence immunoglobulin E (IgE) assay probe 
      based on a DNA aptamer. A Texas red-labeled short DNA strand (T-DNA) 
      complementary with part of the IgE aptamer sequence was used to produce the 
      fluorescence enhancement effected upon the binding of IgE to the aptamer. Another 
      short DNA strand labeled with dabcyl quencher (Q-DNA) complementary with part of 
      the aptamer sequence nearby the T-DNA location was used to lower the background 
      fluorescence. The IgE can be detected in the concentration range from 9.2 x 
      10(-11) to 3.7 x 10(-8) mol L(-1) with a detection limit of 5.7 x 10(-11) mol 
      L(-1).
FAU - He, Jing-Lin
AU  - He JL
AD  - State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry 
      and Chemical Engineering, Hunan University, Changsha 410082, PR China. 
      rqyu@hnu.cn.
FAU - Wu, Zai-Sheng
AU  - Wu ZS
FAU - Zhang, Song-Bai
AU  - Zhang SB
FAU - Shen, Guo-Li
AU  - Shen GL
FAU - Yu, Ru-Qin
AU  - Yu RQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090312
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques
MH  - DNA/*chemistry
MH  - *Fluorescence
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*chemistry
MH  - Protein Binding
MH  - Sensitivity and Specificity
EDAT- 2009/04/22 09:00
MHDA- 2010/03/05 06:00
CRDT- 2009/04/22 09:00
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2010/03/05 06:00 [medline]
AID - 10.1039/b812450g [doi]
PST - ppublish
SO  - Analyst. 2009 May;134(5):1003-7. doi: 10.1039/b812450g. Epub 2009 Mar 12.