PMID- 19381614
OWN - NLM
STAT- MEDLINE
DCOM- 20091125
LR  - 20121115
IS  - 1432-0738 (Electronic)
IS  - 0340-5761 (Linking)
VI  - 83
IP  - 10
DP  - 2009 Oct
TI  - Appraisal of the sensitising potential of orally and dermally administered 
      mercaptobenzothiazole by a biphasic protocol of the local lymph node assay.
PG  - 933-9
LID - 10.1007/s00204-009-0426-y [doi]
AB  - Mercaptobenzothiazole (MBT) is used while manufacturing natural rubber products. 
      Our study deals with assessing its allergenic potential following dermal and oral 
      routes of exposure, using a biphasic local lymph node assay (LLNA). Female Balb/c 
      mice were treated with MBT (dermally 3, 10, 30% concentrations in DMSO; orally 1, 
      10, 100 mg/kg doses in corn oil) on the back (dermal study) or through oral 
      administration (oral study) on days 1-3 followed by auricular application of 3, 
      10 and 30% concentrations, respectively, on days 15-17. End points determined on 
      day 19 included ear thickness, ear punch weight, lymph node weight, lymph node 
      cell count, and lymphocyte subpopulations (CD4+, CD8+, CD45+). After dermal 
      application of 3% or 10% solution, a significant increase in cell count and lymph 
      node weight along with significant decrease in CD8+ cells was observed. After 
      initial oral administration of 1 mg/kg, we noticed a significant amplification in 
      cell count. Following oral administration of 10 mg/kg, we observed a similar 
      increase in cell count and lymph node weight. The results of our study show that 
      the modified biphasic LLNA protocol can be used to study the sensitising 
      potential of a compound also following the oral route of exposure.
FAU - Ahuja, Varun
AU  - Ahuja V
AD  - Institute of Clinical Pharmacology and Toxicology, Charite Medical University, 
      Campus Benjamin Franklin, Garystrasse 5, 14195 Berlin, Germany.
FAU - Wanner, Reinhard
AU  - Wanner R
FAU - Platzek, Thomas
AU  - Platzek T
FAU - Stahlmann, Ralf
AU  - Stahlmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090419
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Allergens)
RN  - 0 (Benzothiazoles)
RN  - 5RLR54Z22K (captax)
SB  - IM
MH  - Administration, Cutaneous
MH  - Administration, Oral
MH  - Allergens/*administration & dosage/immunology
MH  - Animals
MH  - Benzothiazoles/*administration & dosage/*immunology
MH  - CD4-Positive T-Lymphocytes/metabolism
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - Cell Count
MH  - Dermatitis, Allergic Contact/immunology
MH  - Female
MH  - Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2009/04/22 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/04/22 09:00
PHST- 2009/01/21 00:00 [received]
PHST- 2009/03/24 00:00 [accepted]
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1007/s00204-009-0426-y [doi]
PST - ppublish
SO  - Arch Toxicol. 2009 Oct;83(10):933-9. doi: 10.1007/s00204-009-0426-y. Epub 2009 
      Apr 19.