PMID- 19381781
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20221207
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 330
IP  - 1-2
DP  - 2009 Oct
TI  - Association of -394C>G and -420C>G polymorphisms in the RETN gene with T2DM and 
      CHD and a new potential SNP might be exist in exon 3 of RETN gene in Chinese.
PG  - 31-8
LID - 10.1007/s11010-009-0097-2 [doi]
AB  - Genetic variations of the human RETN gene are associated with metabolic 
      phenotypes, including obesity, insulin sensitivity, diabetes, and coronary heart 
      disease (CHD). There are few studies of two gene variants, -394C>G and -420C>G, 
      in Chinese population. This study investigated the distribution of RETN gene, 
      single nucleotide polymorphisms (SNPs), in Chinese Han population and the 
      association of the polymorphisms with type 2 diabetes mellitus (T2DM) and CHD. In 
      a population-based, case-control genetic association study, a total of 961 
      subjects were recruited from the community, including 318 T2DM patients, 273 CHD 
      patients, and 370 unrelated healthy control individuals. Serum lipid levels were 
      detected. Two SNPs of RETN gene, -394C>G and -420C>G, were genotyped by PCR-RFLP. 
      Unknown Polymorphisms were screened with the technique of denaturing high 
      performance liquid chromatography (DHPLC). The frequencies of RETN -394G allele 
      in T2DM group, CHD group, and control group were 0.3066, 0.3555, and 0.3481, 
      respectively, which are met with the Hardy-Weinberg equilibrium. There is a 
      significant difference of the comparison of sex in T2DM group of RETN gene 
      SNP-394C>G (P < 0.05). Compared with controls, there was no significant 
      difference in the distribution of genotypes and allele frequencies of -394C>G 
      polymorphic site in T2DM patients and CHD patients, respectively. No direct 
      association was found between the -394C>G polymorphism and T2DM or CHD. The 
      frequencies of RETN -420G allele in T2DM group, CHD group, and control group were 
      0.4009, 0.3725, and 0.3859, respectively, which are met with the Hardy-Weinberg 
      equilibrium. The frequencies of RETN -420G allele in T2DM groups and control 
      groups of Chinese population are significantly different from those in European 
      population (0.40 vs. 0.27, 0.39 vs. 0.26) (P < 0.01). Compared with controls, 
      there was no significant difference in distribution of genotypes and allele 
      frequencies of -420C>G polymorphic site in T2DM patients and CHD patients, 
      respectively. No direct association was found between the -420C>G polymorphism 
      and T2DM or CHD. In addition, we found new potential SNP +593G>C in exon 3 of 
      RETN gene using DHPLC. The RETN gene exhibits sex and ethnic differences. +593G>C 
      of RETN gene might be a new potential SNP in exon 3 of RETN gene. Association 
      between SNP -394C>G and -420C>G of RETN gene with T2DM and CHD in Chinese needs 
      more exploration.
FAU - Chi, Shuguang
AU  - Chi S
AD  - Department of Cell Biology and Genetics, and Center of Neuroscience, Fujian 
      Medical University, 88 Jiaotong Road, Fuzhou, 350004, China.
FAU - Lan, Cailian
AU  - Lan C
FAU - Zhang, Sizhong
AU  - Zhang S
FAU - Liu, Hekun
AU  - Liu H
FAU - Wang, Xizhen
AU  - Wang X
FAU - Chen, Yuanzhong
AU  - Chen Y
FAU - Chen, Xuexiang
AU  - Chen X
FAU - Chen, Suyun
AU  - Chen S
FAU - Zhang, Wei
AU  - Zhang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090421
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Lipids)
RN  - 0 (RETN protein, human)
RN  - 0 (Resistin)
SB  - IM
MH  - Aged
MH  - Asian People/genetics
MH  - Case-Control Studies
MH  - Coronary Disease/epidemiology/*genetics
MH  - Diabetes Mellitus, Type 2/epidemiology/*genetics
MH  - Exons
MH  - Female
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Lipids/blood
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Resistin/*genetics
MH  - Sex Factors
EDAT- 2009/04/22 09:00
MHDA- 2010/03/03 06:00
CRDT- 2009/04/22 09:00
PHST- 2008/12/12 00:00 [received]
PHST- 2009/03/30 00:00 [accepted]
PHST- 2009/04/22 09:00 [entrez]
PHST- 2009/04/22 09:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - 10.1007/s11010-009-0097-2 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2009 Oct;330(1-2):31-8. doi: 10.1007/s11010-009-0097-2. Epub 
      2009 Apr 21.