PMID- 19384158
OWN - NLM
STAT- MEDLINE
DCOM- 20090514
LR  - 20171116
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 87
IP  - 8
DP  - 2009 Apr 27
TI  - Extracorporeal photopheresis efficiently impairs the proinflammatory capacity of 
      human 6-sulfo LacNAc dendritic cells.
PG  - 1134-9
LID - 10.1097/TP.0b013e31819e02d4 [doi]
AB  - BACKGROUND: Extracorporeal photopheresis (ECP) emerged as a promising treatment 
      modality for steroid-refractory graft-versus-host disease (GVHD), which 
      represents a major complication of allogeneic hematopoietic stem-cell 
      transplantation. Dendritic cells (DCs) display an extraordinary capacity to 
      induce T-cell responses and play a crucial role in the initiation and 
      maintainance of GVHD. This study evaluated the direct impact of ECP on the 
      proinflammatory capacity of 6-sulfo LacNAc (slan) DCs, representing a major 
      subpopulation of human blood DCs. METHODS: SlanDCs were isolated from ECP-treated 
      or untreated blood of healthy donors or GVHD patients by immunomagnetic 
      isolation. The maturation of slanDC was determined by flow cytometry. Cytokine 
      production of slanDCs was measured by enzyme-linked immunosorbent assay. 
      SlanDC-mediated T-cell proliferation was evaluated by H-thymidine incorporation. 
      SlanDC-mediated T-cell programming was determined by flow cytometry. RESULTS: ECP 
      efficiently impairs the spontaneous maturation and secretion of proinflammatory 
      tumor necrosis factor-alpha, interleukin-1beta, and interleukin-12 by slanDCs. 
      Furthermore, ECP markedly inhibits slanDC-induced proliferation of CD4 and CD8 T 
      cells and polarization of naive CD4 T lymphocytes into Th1 cells. CONCLUSIONS: 
      These novel findings indicate that ECP efficiently impairs the proinflammatory 
      capacity of slanDCs, which may represent an important mechanism for the 
      therapeutic efficiency of ECP in GVHD.
FAU - Gerner, Michael
AU  - Gerner M
AD  - Institute of Immunology, Medical Faculty, Technical University of Dresden, 
      Dresden, Germany.
FAU - Holig, Kristina
AU  - Holig K
FAU - Wehner, Rebekka
AU  - Wehner R
FAU - Zhao, Senming
AU  - Zhao S
FAU - Schakel, Knut
AU  - Schakel K
FAU - Bachmann, Michael Philipp
AU  - Bachmann MP
FAU - Rieber, Ernst Peter
AU  - Rieber EP
FAU - Bornhauser, Martin
AU  - Bornhauser M
FAU - Schmitz, Marc
AU  - Schmitz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (6-sulfo-LacNac)
RN  - 0 (ABO Blood-Group System)
RN  - 0 (Amino Sugars)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (PLVAP protein, human)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Proteome)
RN  - 0 (von Willebrand Factor)
SB  - IM
MH  - ABO Blood-Group System/*analysis/immunology
MH  - Amino Sugars/*analysis
MH  - Carrier Proteins/analysis/immunology
MH  - Dendritic Cells/*physiology/radiation effects
MH  - Humans
MH  - Immunoblotting
MH  - Inflammation/*physiopathology/prevention & control
MH  - Membrane Proteins/analysis/immunology
MH  - *Photopheresis
MH  - Platelet Endothelial Cell Adhesion Molecule-1/analysis/immunology
MH  - Proteome
MH  - von Willebrand Factor/analysis/immunology
EDAT- 2009/04/23 09:00
MHDA- 2009/05/15 09:00
CRDT- 2009/04/23 09:00
PHST- 2009/04/23 09:00 [entrez]
PHST- 2009/04/23 09:00 [pubmed]
PHST- 2009/05/15 09:00 [medline]
AID - 00007890-200904270-00006 [pii]
AID - 10.1097/TP.0b013e31819e02d4 [doi]
PST - ppublish
SO  - Transplantation. 2009 Apr 27;87(8):1134-9. doi: 10.1097/TP.0b013e31819e02d4.