PMID- 19384471
OWN - NLM
STAT- MEDLINE
DCOM- 20090817
LR  - 20211020
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Linking)
VI  - 26
IP  - 7
DP  - 2009 Jul
TI  - PLGA and PHBV microsphere formulations and solid-state characterization: possible 
      implications for local delivery of fusidic acid for the treatment and prevention 
      of orthopaedic infections.
PG  - 1644-56
LID - 10.1007/s11095-009-9875-5 [doi]
AB  - PURPOSE: To develop and characterize the solid-state properties of 
      poly(DL-lactic-co-glycolic acid) (PLGA) and poly(3-hydroxybutyric 
      acid-co-3-hydroxyvaleric acid) (PHBV) microspheres for the localized and 
      controlled release of fusidic acid (FA). METHODS: The effects of FA loading and 
      polymer composition on the mean diameter, encapsulation efficiency and FA 
      released from the microspheres were determined. The solid-state and phase 
      separation properties of the microspheres were characterized using DSC, XRPD, 
      Raman spectroscopy, SEM, laser confocal and real time recording of single 
      microspheres formation. RESULTS: Above a loading of 1% (w/w) FA phase separated 
      from PLGA polymer and formed distinct spherical FA-rich amorphous microdomains 
      throughout the PLGA microsphere. For FA-loaded PLGA microspheres, encapsulation 
      efficiency and cumulative release increased with initial drug loading. Similarly, 
      cumulative release from FA-loaded PHBV microspheres was increased by FA loading. 
      After the initial burst release, FA was released from PLGA microspheres much 
      slower compared to PHBV microspheres. CONCLUSIONS: A unique phase separation 
      phenomenon of FA in PLGA but not in PHBV polymers was observed, driven by 
      coalescence of liquid microdroplets of a DCM-FA-rich phase in the forming 
      microsphere.
FAU - Yang, Chiming
AU  - Yang C
AD  - Faculty of Pharmaceutical Sciences, The University of British Columbia, 2146 East 
      Mall, Vancouver BCV6T1Z3, Canada.
FAU - Plackett, David
AU  - Plackett D
FAU - Needham, David
AU  - Needham D
FAU - Burt, Helen M
AU  - Burt HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090421
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Glycolates)
RN  - 0 (Polyesters)
RN  - 0 (poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate))
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 59XE10C19C (Fusidic Acid)
SB  - IM
MH  - Anti-Bacterial Agents/*chemistry
MH  - Calorimetry, Differential Scanning
MH  - Delayed-Action Preparations/*chemistry
MH  - Fusidic Acid/*chemistry
MH  - Glycolates/*chemistry
MH  - Kinetics
MH  - Lactic Acid
MH  - *Microspheres
MH  - Particle Size
MH  - Phase Transition
MH  - Polyesters/*chemistry
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Powder Diffraction
MH  - Surface Properties
MH  - X-Ray Diffraction
EDAT- 2009/04/23 09:00
MHDA- 2009/08/18 09:00
CRDT- 2009/04/23 09:00
PHST- 2008/09/23 00:00 [received]
PHST- 2009/03/16 00:00 [accepted]
PHST- 2009/04/23 09:00 [entrez]
PHST- 2009/04/23 09:00 [pubmed]
PHST- 2009/08/18 09:00 [medline]
AID - 10.1007/s11095-009-9875-5 [doi]
PST - ppublish
SO  - Pharm Res. 2009 Jul;26(7):1644-56. doi: 10.1007/s11095-009-9875-5. Epub 2009 Apr 
      21.