PMID- 19387587
OWN - NLM
STAT- MEDLINE
DCOM- 20090520
LR  - 20090423
IS  - 0253-6269 (Print)
IS  - 0253-6269 (Linking)
VI  - 32
IP  - 3
DP  - 2009 Mar
TI  - A novel in vitro ischemia/reperfusion injury model.
PG  - 421-9
LID - 10.1007/s12272-009-1316-9 [doi]
AB  - The reperfusion of blood flow occurred in a number of conditions such as stroke 
      and organ transplantation immensely augments tissue injury and causes more severe 
      damage than prolonged ischemia. In the present study, we designed a novel 
      double-layer parallel-plate flow chamber (PPFC) to develop an in vitro 
      ischemia/reperfusion (I/R) injury model and examined the effects of I/R on 
      inflammatory responses in human microvascular endothelial cells (HMEC-1). The 
      expression of pro-inflammatory mediators, such as interleukin-6 (IL-6), monocyte 
      chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1), 
      E-selectin, and vascular cell adhesion molecule-1 (VCAM-1) in HMEC-1 was measured 
      by quantitative real-time RT-PCR. The cells were also pre-treated with 
      antioxidant pyrrolidine dithiocarbamate (PDTC) to verify involvement of an 
      oxidative mechanism in I/R injury in vitro. The morphological changes and 
      attenuated expression of pro-inflammatory mediators were observed in HMCE-1 
      exposed to the physiological flow. In contrast, I/R markedly and significantly 
      up-regulated expression of pro-inflammatory mediators in HMEC-1. Additionally, 
      pretreatment with PDTC significantly reduced I/R-mediated overexpression of 
      pro-inflammatory mediators. The data from the present study provide evidence 
      demonstrating that our newly designed PPFC can be utilized as an effective in 
      vitro cell culture model system to develop new drugs specifically targeting 
      against ischemia/reperfusion (I/R) injury.
FAU - Lee, Won Hee
AU  - Lee WH
AD  - School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and 
      State University (Virginia Tech), Blacksburg, VA 24061, USA.
FAU - Kang, Sungkwon
AU  - Kang S
FAU - Vlachos, Pavlos P
AU  - Vlachos PP
FAU - Lee, Yong Woo
AU  - Lee YW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090423
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Antioxidants)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Pyrrolidines)
RN  - 0 (Thiocarbamates)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - *Cell Culture Techniques
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Gene Expression Regulation
MH  - *Hemorheology
MH  - Humans
MH  - Inflammation/genetics/*metabolism/physiopathology
MH  - Inflammation Mediators/*metabolism
MH  - Microcirculation
MH  - Oxidative Stress
MH  - Pyrrolidines/pharmacology
MH  - Regional Blood Flow
MH  - Reperfusion Injury/genetics/*metabolism/physiopathology
MH  - Stress, Mechanical
MH  - Thiocarbamates/pharmacology
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2009/04/24 09:00
MHDA- 2009/05/21 09:00
CRDT- 2009/04/24 09:00
PHST- 2008/11/12 00:00 [received]
PHST- 2009/03/06 00:00 [accepted]
PHST- 2009/03/04 00:00 [revised]
PHST- 2009/04/24 09:00 [entrez]
PHST- 2009/04/24 09:00 [pubmed]
PHST- 2009/05/21 09:00 [medline]
AID - 10.1007/s12272-009-1316-9 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2009 Mar;32(3):421-9. doi: 10.1007/s12272-009-1316-9. Epub 2009 
      Apr 23.