PMID- 19388090
OWN - NLM
STAT- MEDLINE
DCOM- 20091202
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 91
IP  - 1
DP  - 2009 Oct
TI  - RGD modified PLGA/gelatin microspheres as microcarriers for chondrocyte delivery.
PG  - 228-38
LID - 10.1002/jbm.b.31394 [doi]
AB  - Poly(lactide-co-glycotide) (PLGA)/gelatin composite microspheres were prepared by 
      an emulsion solvent evaporation technique. RGDS peptides were further immobilized 
      under the catalyzation of water soluble carbodiimide (EDAC). Confocal laser 
      scanning microscopy and transmission electron microscopy revealed that the 
      gelatin was entrapped in the PLGA/gelatin microspheres with a manner of separated 
      domains. The contents of the entrapped gelatin and immobilized RGDS peptides were 
      quantified as 0.9 mg/20 mg and approximately 2.1 microg/20 mg microspheres by 
      hydroxyproline analysis and bicinchoninic acid protein assay, respectively. 
      Moreover, difference in morphology of PLGA, PLGA/gelatin and RGDS modified 
      PLGA/gelatin (PLGA/gelatin-RGDS) microspheres was observed by scanning electron 
      microscopy. The PLGA/gelatin and PLGA/gelatin-RGDS microspheres lost their weight 
      rapidly in PBS, but slowly in DMEM/fetal bovine serum. Rabbit auricular 
      chondrocytes were seeded onto the microspheres in vitro to assess their 
      biological performance and applicability as cell carriers. Results show that 
      amongst the PLGA, PLGA/gelatin and PLGA/gelatin-RGDS microspheres, the latter 
      ones have the best performance in terms of chondrocyte attachment, proliferation, 
      viability and sulfated glycosaminoglycans secretion.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Tan, Huaping
AU  - Tan H
AD  - Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of 
      Education, Department of Polymer Science and Engineering, Zhejiang University, 
      Hangzhou 310027, China.
FAU - Huang, Dejuan
AU  - Huang D
FAU - Lao, Lihong
AU  - Lao L
FAU - Gao, Changyou
AU  - Gao C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Drug Carriers)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Oligopeptides)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/metabolism
MH  - Drug Carriers/*chemistry
MH  - Gelatin/*chemistry
MH  - Glycosaminoglycans/chemistry/metabolism
MH  - Lactic Acid/*chemistry
MH  - Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - *Microspheres
MH  - Oligopeptides/*chemistry
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rabbits
EDAT- 2009/04/24 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/04/24 09:00
PHST- 2009/04/24 09:00 [entrez]
PHST- 2009/04/24 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1002/jbm.b.31394 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2009 Oct;91(1):228-38. doi: 
      10.1002/jbm.b.31394.