PMID- 19389081
OWN - NLM
STAT- MEDLINE
DCOM- 20091019
LR  - 20191210
IS  - 1865-1674 (Print)
IS  - 1865-1674 (Linking)
VI  - 56
IP  - 6-7
DP  - 2009 Aug
TI  - Evaluation of granulysin and perforin as candidate biomarkers for protection 
      following vaccination with Mycobacterium bovis BCG or M. bovisDeltaRD1.
PG  - 228-39
LID - 10.1111/j.1865-1682.2008.01058.x [doi]
AB  - The development of improved vaccines against tuberculosis (TB) is directly linked 
      to the investigation of new and better correlates of protection after vaccination 
      against TB. Cloning and characterization of bovine homologues of the 
      antimicrobial protein granulysin (Bo-lysin) and perforin by our group could be 
      used as potential biomarkers for TB vaccination efficacy. In the present study, 
      we examined the kinetics of granulysin, perforin, IFNgamma and Fas-L responses to 
      Mycobacterium bovis purified protein derivative (PPD) stimulation by peripheral 
      blood mononuclear cells from M. bovisDeltaRD1-, BCG- and non-vaccinated cattle. 
      Gene expression profiles following PPD stimulation showed significant increases 
      in transcripts for granulysin and IFNgamma in both CD4(+) and CD8(+) T cells in 
      BCG-vaccinated as compared with non-vaccinated animals. Perforin and IFNgamma 
      examined by flow cytometry, showed a difference of 1-2% more PPD-specific cells 
      in BCG-vaccinated than non-vaccinated animals. In the vaccine trial, granulysin 
      and perforin were significantly increased in both vaccine groups as compared with 
      control after vaccination and challenge. IFNgamma expression was increased only 
      after vaccination and secretion was higher in the control, non-protected group as 
      compared with both vaccine groups demonstrating no correlation with protection 
      upon vaccination. In summary, results shown here provide evidence that granulysin 
      and perforin are prospective candidates as biomarkers of protection after 
      vaccination against TB.
FAU - Capinos Scherer, Charles F
AU  - Capinos Scherer CF
AD  - Department of Microbiology and Immunology, University of Texas Medical Branch, 
      Galveston, TX, USA.
FAU - Endsley, Janice J
AU  - Endsley JJ
FAU - de Aguiar, Juliana B
AU  - de Aguiar JB
FAU - Jacobs, William R Jr
AU  - Jacobs WR Jr
FAU - Larsen, Michelle H
AU  - Larsen MH
FAU - Palmer, Mitchell V
AU  - Palmer MV
FAU - Nonnecke, Brian J
AU  - Nonnecke BJ
FAU - Ray Waters, W
AU  - Ray Waters W
FAU - Mark Estes, D
AU  - Mark Estes D
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090311
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (BCG Vaccine)
RN  - 0 (Biomarkers)
RN  - 126465-35-8 (Perforin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Antigens, Differentiation, T-Lymphocyte/biosynthesis/*blood/genetics
MH  - BCG Vaccine/*immunology
MH  - Biomarkers/blood
MH  - Cattle/*immunology
MH  - Gene Expression Profiling
MH  - Interferon-gamma/biosynthesis/blood/genetics
MH  - Lymphocytes/immunology
MH  - Mycobacterium bovis/genetics/*immunology
MH  - Perforin/biosynthesis/*blood/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tuberculosis, Bovine/*prevention & control
EDAT- 2009/04/25 09:00
MHDA- 2009/10/20 06:00
CRDT- 2009/04/25 09:00
PHST- 2009/04/25 09:00 [entrez]
PHST- 2009/04/25 09:00 [pubmed]
PHST- 2009/10/20 06:00 [medline]
AID - JVA1058 [pii]
AID - 10.1111/j.1865-1682.2008.01058.x [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2009 Aug;56(6-7):228-39. doi: 
      10.1111/j.1865-1682.2008.01058.x. Epub 2009 Mar 11.