PMID- 19389097
OWN - NLM
STAT- MEDLINE
DCOM- 20090609
LR  - 20090520
IS  - 1524-4725 (Electronic)
IS  - 1076-0512 (Linking)
VI  - 35
IP  - 5
DP  - 2009 May
TI  - Intense pulsed light effects on the expression of extracellular matrix proteins 
      and transforming growth factor beta-1 in skin dermal fibroblasts cultured within 
      contracted collagen lattices.
PG  - 816-25
LID - 10.1111/j.1524-4725.2009.01138.x [doi]
AB  - BACKGROUND: Emerging clinical evidence suggests that intense pulsed light (IPL) 
      treatment may exert some beneficial effects on photoaged skin. The molecular 
      mechanisms underlying this IPL effect have not been fully elucidated. OBJECTIVE: 
      To examine the effects of IPL irradiation on normal human dermal fibroblasts 
      grown in contracted collagen lattices. METHODS: Human skin fibroblasts cultured 
      in contracted collagen lattices were irradiated with IPL with triple pulses of 7 
      ms with a pulse interval of 70 ms and fluences of 20, 50, and 75 J/cm(2). 
      Twenty-four hours after the irradiation, cell viability, messenger RNA (mRNA), 
      and protein levels of extracellular matrix proteins (e.g., collagen I, collagen 
      III, and fibronectin) and transforming growth factor beta-1 (TGF-beta1) were 
      evaluated using dye exclusion, real-time reverse transcriptase polymerase chain 
      reaction, and enzyme-linked immunosorbent assay, respectively. RESULTS: A 
      dose-dependent increase in viable cells was demonstrated after the IPL 
      irradiation. There was no significant change in mRNA levels of collagen I and 
      fibronectin. Upregulated expression of collagen III and TGF-beta1 in dermal 
      fibroblasts was verified. CONCLUSIONS: The analytical results presented here 
      provide a potential mechanistic explanation for the mechanism of clinical 
      photorejuvenation effects of IPL that involves the increase of extracellular 
      matrix construction by upregulating the gene expressions of collagen III and 
      TGF-beta1.
FAU - Wong, Wen-Rou
AU  - Wong WR
AD  - Graduate Institute of Clinical Medical Sciences, Chang Gung Memorial Hospital, 
      Taipei, Taiwan.
FAU - Shyu, Wen-Ling
AU  - Shyu WL
FAU - Tsai, Jung-Wei
AU  - Tsai JW
FAU - Hsu, Kuang-Hung
AU  - Hsu KH
FAU - Pang, Jong-Hwei S
AU  - Pang JH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090330
PL  - United States
TA  - Dermatol Surg
JT  - Dermatologic surgery : official publication for American Society for Dermatologic 
      Surgery [et al.]
JID - 9504371
RN  - 0 (Collagen Type III)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Cells, Cultured
MH  - Collagen Type III/biosynthesis/*genetics
MH  - Culture Media, Conditioned
MH  - Dermis/cytology/*metabolism/radiation effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibroblasts/cytology/*metabolism/radiation effects
MH  - Gene Expression Regulation/*radiation effects
MH  - Humans
MH  - Phototherapy/*methods
MH  - RNA/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transforming Growth Factor beta1/biosynthesis/*genetics
EDAT- 2009/04/25 09:00
MHDA- 2009/06/10 09:00
CRDT- 2009/04/25 09:00
PHST- 2009/04/25 09:00 [entrez]
PHST- 2009/04/25 09:00 [pubmed]
PHST- 2009/06/10 09:00 [medline]
AID - DSU1138 [pii]
AID - 10.1111/j.1524-4725.2009.01138.x [doi]
PST - ppublish
SO  - Dermatol Surg. 2009 May;35(5):816-25. doi: 10.1111/j.1524-4725.2009.01138.x. Epub 
      2009 Mar 30.