PMID- 19389276
OWN - NLM
STAT- MEDLINE
DCOM- 20090526
LR  - 20211020
IS  - 1743-2928 (Electronic)
IS  - 1351-0002 (Print)
IS  - 1351-0002 (Linking)
VI  - 14
IP  - 2
DP  - 2009
TI  - Hydroxyl radical oxidation of guanosine 5'-triphosphate (GTP): requirement for a 
      GTP-Cu(II) complex.
PG  - 82-92
LID - 10.1179/135100009X392520 [doi]
AB  - Levels of oxidized guanosine base in DNA have become a hallmark biomarker in 
      assessing oxidative stress implicated in a variety of disease and toxin-induced 
      states. However, there is evidence that the guanosine in the nucleotide 
      triphosphate pool (GTP) is more susceptible to oxidation than guanosine residues 
      incorporated into nucleic acids and this causes a substantial amount of the 
      oxidized product, 8-oxoguanosine 5'-triphosphate (oxo(8)GTP), to accumulate in 
      cell-free and in cell-culture preparations. Electron paramagnetic resonance (EPR) 
      spectroscopy and direct EPR analysis of free radical production by copper sulfate 
      and L-ascorbic acid demonstrates that the hydroxyl radical (HO(*)) is produced 
      via oxidation of Cu(+) to Cu(2+) while in a complex with GTP. This HO(*) 
      production is dependent on the availability of oxygen and the presence of GTP in 
      the reaction milieu. Verification of free radical-mediated production of 
      oxo(8)GTP is presented using HPLC with electrochemical detection and 
      matrix-assisted laser desorption/ionization linear time-of-flight mass 
      spectrometry (MALDI-LTOF-MS). The sum of these results is presented in a novel 
      mechanism of GTP oxidation by Cu(2+) and L-ascorbic acid. A better understanding 
      of the chemistry involved in this oxidative modification of GTP facilitates a 
      more comprehensive understanding of its potential physiological consequences.
FAU - Cerchiaro, Giselle
AU  - Cerchiaro G
AD  - Centro de Ciencias Naturais e Humanas, Universidade Federal do ABC, Santo 
      Andre-SP, Brazil.
FAU - Bolin, Celeste
AU  - Bolin C
FAU - Cardozo-Pelaez, Fernando
AU  - Cardozo-Pelaez F
LA  - eng
GR  - 1R15 AG 023604-01/AG/NIA NIH HHS/United States
GR  - P20 RR017670-04/RR/NCRR NIH HHS/United States
GR  - P20 RR 015583-07/RR/NCRR NIH HHS/United States
GR  - R15 AG023604-01/AG/NIA NIH HHS/United States
GR  - P30 GM103338/GM/NIGMS NIH HHS/United States
GR  - P20 RR015583/RR/NCRR NIH HHS/United States
GR  - P20 RR 017670-04/RR/NCRR NIH HHS/United States
GR  - P20 RR017670/RR/NCRR NIH HHS/United States
GR  - P20 RR015583-07/RR/NCRR NIH HHS/United States
GR  - R15 AG023604/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Redox Rep
JT  - Redox report : communications in free radical research
JID - 9511366
RN  - 0 (Reactive Oxygen Species)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 789U1901C5 (Copper)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 9007-49-2 (DNA)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Ascorbic Acid/chemistry
MH  - Copper/*chemistry
MH  - DNA/chemistry/metabolism
MH  - Electron Spin Resonance Spectroscopy
MH  - Guanosine Triphosphate/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Hydroxyl Radical/*chemistry
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Oxygen Consumption
MH  - Reactive Oxygen Species/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PMC - PMC2759603
MID - NIHMS137529
EDAT- 2009/04/25 09:00
MHDA- 2009/05/27 09:00
PMCR- 2010/01/01
CRDT- 2009/04/25 09:00
PHST- 2009/04/25 09:00 [entrez]
PHST- 2009/04/25 09:00 [pubmed]
PHST- 2009/05/27 09:00 [medline]
PHST- 2010/01/01 00:00 [pmc-release]
AID - 10.1179/135100009X392520 [doi]
PST - ppublish
SO  - Redox Rep. 2009;14(2):82-92. doi: 10.1179/135100009X392520.