PMID- 19389475
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20211203
IS  - 1878-5492 (Electronic)
IS  - 0966-3274 (Linking)
VI  - 21
IP  - 3
DP  - 2009 Jul
TI  - Anti-CD(45)RB monoclonal antibody induces immunologic toleration by suppressing 
      dendritic cells.
PG  - 136-9
LID - 10.1016/j.trim.2009.04.001 [doi]
AB  - BACKGROUND: In transplantation, the antigen presentation step of T cell 
      activation determines whether the T cell rejects the graft, ignores the graft, or 
      inhibits other T cells from attacking the graft. Dendritic cells (DCs) are the 
      most potent antigen presenting cells that are capable of activating naive T 
      cells. The purpose of the present study was to investigate the influence of 
      anti-CD(45)RB monoclonal antibody on the phenotypes and functions of DCs and to 
      further illustrate the mechanism of anti-CD(45)RB monoclonal antibody-inducing 
      immunologic tolerance. METHODS: DCs from mice were cultured and treated with 
      varying doses of anti-CD(45)RB monoclonal antibody at specific time points. 
      Fluorescence-activated cell sorting (FACS) was used to verify the changes of 
      phenotypes in the cultured DCs labeled with fluorescent antibody. DCs were also 
      used as stimulators in mixed leukocyte reaction to detect their ability to 
      stimulate T cell proliferation. RESULTS: Anti-CD(45)RB monoclonal antibody 
      induced a dose- and time-dependent suppression of maturation of DCs and a 
      dose-dependent suppression of T cell proliferation in mixed leukocyte reaction 
      when anti-CD(45)RB monoclonal antibody-treated DCs were used as stimulators. 
      CONCLUSIONS: One of the mechanisms of anti-CD(45)RB monoclonal antibody-induced 
      tolerance was inhibiting maturation and function of DCs and inducing tolerogenic 
      DCs to achieve a new immune balance.
FAU - Xia, Xiaoping
AU  - Xia X
AD  - Department of Ophthalmology, 3rd Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, China.
FAU - Zhang, Xiao
AU  - Zhang X
FAU - Huang, Xuyan
AU  - Huang X
FAU - Ichim, Thomas E
AU  - Ichim TE
FAU - Li, Mu
AU  - Li M
FAU - Luke, Patrick
AU  - Luke P
FAU - Min, Weiping
AU  - Min W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090421
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immunologic Factors)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/administration & dosage/*immunology
MH  - Antigen Presentation
MH  - Dendritic Cells/*immunology
MH  - *Immune Tolerance
MH  - Immunologic Factors/administration & dosage/*immunology
MH  - Immunosuppression Therapy/*methods
MH  - Leukocyte Common Antigens/*antagonists & inhibitors/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
EDAT- 2009/04/25 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/04/25 09:00
PHST- 2008/12/29 00:00 [received]
PHST- 2009/04/07 00:00 [revised]
PHST- 2009/04/10 00:00 [accepted]
PHST- 2009/04/25 09:00 [entrez]
PHST- 2009/04/25 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
AID - S0966-3274(09)00038-0 [pii]
AID - 10.1016/j.trim.2009.04.001 [doi]
PST - ppublish
SO  - Transpl Immunol. 2009 Jul;21(3):136-9. doi: 10.1016/j.trim.2009.04.001. Epub 2009 
      Apr 21.